| Size | Price | Stock | Qty |
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| 1mg |
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| Other Sizes |
| Targets |
PROTAC BRD4 ligand-2 hydrochloride targets the BRD4 protein, a key epigenetic reader. This ligand is specifically designed for use with the PROTAC CFT-2718. Substituting it with other BRD4 ligands would alter the ternary complex geometry, E3 ligase recruitment efficiency, and degradation profile. It is a ligand for target BRD4 protein for PROTAC CFT-2718.
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| ln Vitro |
PROTAC BRD4 ligand-2 hydrochloride is a ligand for BRD4. Its primary activity is its ability to bind to BRD4. When incorporated into a PROTAC, it facilitates the formation of a ternary complex and subsequent degradation of BRD4.
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| ln Vivo |
PROTAC BRD4 ligand-2 hydrochloride is a research chemical used as a building block for PROTAC synthesis. PROTACs synthesized using this ligand, such as CFT-2718, have been evaluated in preclinical models of small-cell lung cancer and pancreatic cancer for their ability to degrade BRD4 and inhibit tumor growth.
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| Enzyme Assay |
A cell-free binding assay for PROTAC BRD4 ligand-2 would use SPR. Recombinant BRD4 protein is immobilized on a sensor chip. Increasing concentrations of PROTAC BRD4 ligand-2 hydrochloride are flowed over the chip. Alternatively, a TR-FRET-based competition assay can be used. The compound is typically dissolved in DMSO. The Kd value is calculated from the binding curve. This ligand is specifically designed for use with PROTAC CFT-2718.
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| Cell Assay |
PROTAC BRD4 ligand-2 hydrochloride is a small molecule for PROTAC synthesis. It is not used in a typical cell-based assay without being conjugated into a full PROTAC. To assess its cellular activity, it is first conjugated to an E3 ligase ligand to form a PROTAC degrader. This PROTAC is then used in cell-based assays to measure BRD4 protein levels by Western blot and cell viability.
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| Animal Protocol |
A standard in vivo animal protocol for a BRD4 PROTAC would involve mouse xenograft models. Female BALB/c nude mice are implanted subcutaneously with cancer cells (e.g., SCLC or pancreatic cancer cells). When tumors reach 100-150 mm3, mice are randomized and treated with the PROTAC (e.g., CFT-2718). Tumor volume and body weight are monitored. BRD4 degradation in tumors is confirmed by Western blot or IHC.
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| ADME/Pharmacokinetics |
PROTAC BRD4 ligand-2 hydrochloride has a molecular weight of 437.32 and a molecular formula of C22H18Cl2N6. It is a powder that should be stored at -20degC for up to 3 years. In solution, it is stable for 1 year at -80degC. The hydrochloride salt enhances solubility. Specific PK data for the ligand alone are not available.
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| Toxicity/Toxicokinetics |
As a research chemical, no toxicity data are available for PROTAC BRD4 ligand-2 hydrochloride. It should be handled with standard laboratory safety precautions, using PPE and in a well-ventilated area. The small quantities used in research are unlikely to pose a significant acute hazard.
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| References | |
| Additional Infomation |
PROTAC BRD4 ligand-2 hydrochloride is the hydrochloride salt of a BRD4-binding small molecule. It is a highly pure research compound intended for biochemical and cellular studies. This compound is not a drug and is not approved for therapeutic use. It is a versatile tool for developing potent BRD4 degraders. At equivalent molar concentrations, the salt and free forms exhibit comparable biological activity, but the salt has better solubility and stability.
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| Molecular Formula |
C22H18CL2N6
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|---|---|
| Molecular Weight |
437.32
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| Related CAS # |
PROTAC BRD4 ligand-2;2154358-11-7
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| Appearance |
Off-white to light yellow solid powder
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO :~100 mg/mL (~228.67 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.72 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.2867 mL | 11.4333 mL | 22.8666 mL | |
| 5 mM | 0.4573 mL | 2.2867 mL | 4.5733 mL | |
| 10 mM | 0.2287 mL | 1.1433 mL | 2.2867 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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