| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
CaMKII
CaMKII (Ca2+/calmodulin-dependent protein kinase II). As a substrate peptide for CaMKII, Syntide 2 does not directly bind to a receptor but is phosphorylated by CaMKII. It shows a Km of 12 uM for CaMKII. It also exhibits a selective inhibitory effect on the gibberellin (GA) response, leaving constitutive and abscisic acid-regulated events unaffected. |
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| ln Vitro |
Syntide 2 (TFA) is a well-characterized in vitro substrate for CaMKII. It is not intended as an inhibitor but rather as a tool for quantifying CaMKII enzymatic activity. The peptide is readily phosphorylated by CaMKII, allowing for the assessment of kinase activity in cell lysates or purified enzyme preparations. The TFA salt form improves its solubility and handling properties.
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| ln Vivo |
In vivo activity for Syntide 2 (TFA) is not reported as the compound is a research reagent used primarily for in vitro kinase assays. It is not a drug candidate or a therapeutic agent but serves as a laboratory tool to study CaMKII function and regulation. The peptide does not have established in vivo efficacy or pharmacokinetic properties for systemic administration.
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| Enzyme Assay |
Kinase activity assay: Purified CaMKII (5-10 ng) is incubated with Syntide 2 (0-1000 uM) as substrate, 50 uM ATP (including [gamma-32P]-ATP), 10 mM MgCl2, 0.5 mM CaCl2, and 50 ug/mL calmodulin in 50 mM HEPES buffer pH 7.5 for 10 min at 30degC. The reaction is terminated by adding phosphoric acid. The reaction mixture is spotted onto P81 phosphocellulose paper, washed with 0.75% phosphoric acid, and radioactivity is measured by scintillation counting.
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| Cell Assay |
Cell lysis and kinase assay: Cultured cells are lysed in RIPA buffer containing protease inhibitors. Lysates (10-50 ug protein) are incubated with Syntide 2 (0-1000 uM) as substrate in kinase reaction buffer (50 mM HEPES, pH 7.5, 10 mM MgCl2, 0.5 mM CaCl2, 50 ug/mL calmodulin, 50 uM ATP) for 15 min at 30degC. The reaction is spotted onto P81 paper, washed, and 32P incorporation is measured. Alternatively, a non-radioactive ELISA-based method detecting phosphorylated peptide can be used.
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| Animal Protocol |
Not applicable. Syntide 2 (TFA) is a peptide substrate used for in vitro biochemical assays and is not administered to animals for therapeutic efficacy testing. It may be used in ex vivo kinase activity assays from tissue lysates derived from treated animals to measure CaMKII activity. The peptide itself is not dosed.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for Syntide 2 (TFA) are not available, as this product is a research reagent for in vitro assays and not a therapeutic drug. It is administered exclusively in cell-free and cell-based systems for kinase activity measurements. As a peptide (MW 1621.84), it is water-soluble (100 mg/mL) and the TFA salt enhances its stability.
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| Toxicity/Toxicokinetics |
Toxicity data for Syntide 2 (TFA) are not reported, as it is a research-grade peptide substrate used for in vitro applications only. It is not intended for human or animal consumption. Standard laboratory safety precautions should be followed when handling synthetic peptides, including the use of gloves and protective eyewear.
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| References | |
| Additional Infomation |
Syntide 2 (TFA) is strictly a research reagent and has not entered clinical trials or been approved for human therapeutic use. It is widely used in signal transduction research to study CaMKII activity. The peptide sequence is PLARTLSVAGLPGKK. The product should be stored as powder at -20degC for long-term stability. For research use only.
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| Molecular Formula |
C70H123N20F3O20
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|---|---|
| Molecular Weight |
1621.84
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| Related CAS # |
Syntide 2;108334-68-5
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| Appearance |
White to off-white solid powder
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O :~100 mg/mL (~61.66 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (61.66 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.6166 mL | 3.0829 mL | 6.1658 mL | |
| 5 mM | 0.1233 mL | 0.6166 mL | 1.2332 mL | |
| 10 mM | 0.0617 mL | 0.3083 mL | 0.6166 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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