| Size | Price | Stock | Qty |
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| 1mg |
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| Other Sizes |
| Targets |
Anti-T7 tag antibody. T7 Tag Peptide is an epitope tag recognized by commercially available anti-T7 tag antibodies. It is not a drug target; rather, it is a tool for detecting and purifying recombinant proteins. The peptide can be used as a blocking reagent, a positive control in Western blots or ELISA, or as a competitor in antibody binding studies.
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| ln Vitro |
In vitro, T7 Tag Peptide is used to confirm the specificity of anti-T7 tag antibodies. It can compete with T7-tagged proteins for antibody binding, allowing validation of antibody specificity. It is not intended to have any biological activity on cells. The TFA salt ensures the peptide is readily soluble in aqueous buffers for assay use.
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| ln Vivo |
Not applicable. T7 Tag Peptide TFA is not administered to animals as a therapeutic or diagnostic agent. It is used exclusively as a laboratory reagent in protein biochemistry and immunoassays. It may be used in animal studies as a positive control for antibody injections if immunized against T7 tag, but this is rare.
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| Enzyme Assay |
T7 tag antibody binding assay (ELISA): A microplate is coated with T7 Tag Peptide TFA (0.1-10 ug/mL) overnight at 4degC. After blocking, increasing concentrations of anti-T7 tag antibody (0-1000 ng/mL) are added and detected with an HRP-labeled secondary antibody. Alternatively, the peptide can be used as a competitor: antibody is pre-incubated with soluble peptide before adding to a plate coated with T7-tagged protein to determine IC50.
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| Cell Assay |
Cell-based protein detection: Cells are transfected with a T7-tagged expression vector. After 24-48 h, cell lysates are prepared. For Western blot, the lysates are electrophoresed and transferred, then the membrane is incubated with anti-T7 tag antibody. A parallel control lane can be treated with T7 Tag Peptide TFA during antibody incubation to confirm band specificity (competitive blocking). For immunoprecipitation, the peptide can be used for elution.
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| Animal Protocol |
Not applicable for direct in vivo use. In some models, T7-tagged proteins are administered to mice, and T7 Tag Peptide TFA can be used to block the tag for control purposes, but this is uncommon. The primary use is in vitro as a biochemical tool.
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| ADME/Pharmacokinetics |
Not applicable. T7 Tag Peptide TFA is a research reagent, not a therapeutic drug. It is soluble in water and common buffers. The TFA salt is used to improve handling and stability. For storage, keep the lyophilized powder at -20degC, desiccated.
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| Toxicity/Toxicokinetics |
The peptide is non-toxic at the concentrations used in vitro (typically 0.1-10 ug/mL). It is not intended for human or animal consumption. Standard safety precautions for handling laboratory peptides should be followed.
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| References |
[1]. Tsutomu Tanaka, et al. A Peptidyl Linker for Protein Cross-Linking Catalyzed by Microbial Transglutaminase. The Society of Chemical Engineers, Japan. Session ID : 3J-11.
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| Additional Infomation |
T7 Tag Peptide TFA is a research-grade epitope tag reagent. It is not a drug and has no therapeutic indications. The sequence is MASMTGGQQMG. It is widely used in molecular biology, protein purification, and detection. For research use only. Store at -20degC, protected from light and moisture.
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| Molecular Formula |
C43H72F3N13O18S3
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| Related CAS # |
T7 Tag Peptide;245445-88-9
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| Appearance |
Typically exists as solid at room temperature
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO :~50 mg/mL (~41.24 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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