Size | Price | Stock | Qty |
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1mg |
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5mg |
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10mg |
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Other Sizes |
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ln Vitro |
Strong mRNA and pDNA entrapment is seen in G0-C14, with an encapsulation effectiveness of greater than 95%[1]. 1. Dissolve PolyHCPT and DSPE-PEG3K in DMF to create a homogeneous solution with a concentration of 5 mg/mL in order to prepare NPs [1]. 2. Create a combination using G0-C14 (5 mg/mL in DMF) and 1 nmol of siRNA (0.1 nmol/µL aqueous solution) at various N/P molar ratios. Combine them with the DSPE-PEG3K solution and polyHCPT. 3. Pour the mixture into 5 milliliters of deionized water dropwise while swirling vigorously (at 1000 rpm). 4. Move the NP dispersion that has produced to an ultrafiltration apparatus. 5. Centrifuge for 8 minutes at room temperature (2800 rpm) in order to eliminate unbound compounds and organic solvent.
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References |
[1]. Li S, et al. Redox-responsive polyprodrug nanoparticles for targeted siRNA delivery and synergistic liver cancer therapy. Biomaterials. 2020 Mar;234:119760.
[2]. Chen Q, et al. Biodegradable nanoparticles decorated with different carbohydrates for efficient macrophage-targeted gene therapy. J Control Release. 2020 Jul 10;323:179-190. |
Molecular Formula |
C106H216N10O10
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Molecular Weight |
1790.91
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CAS # |
1510653-27-6
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SMILES |
C(NCCN(CC(O)CCCCCCCCCCCC)CC(O)CCCCCCCCCCCC)(=O)CCN(CCC(NCCNCC(O)CCCCCCCCCCCC)=O)CCN(CCC(NCCNCC(O)CCCCCCCCCCCC)=O)CCC(=O)NCCN(CC(O)CCCCCCCCCCCC)CC(O)CCCCCCCCCCCC
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO: 100 mg/mL (55.84 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (1.40 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (1.40 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: 2.5 mg/mL (1.40 mM) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 0.5584 mL | 2.7919 mL | 5.5838 mL | |
5 mM | 0.1117 mL | 0.5584 mL | 1.1168 mL | |
10 mM | 0.0558 mL | 0.2792 mL | 0.5584 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.