| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
The primary target of DLPC is the orphan nuclear receptor liver receptor homolog-1 (LRH-1, also known as NR5A2), which is a critical regulator of bile salt biosynthesis. DLPC acts as a selective agonist ligand for this receptor in vitro. Other nuclear receptors including FXR, CAR, PXR, PPARα, and PPARγ are all unaffected by DLPC in cell culture, indicating specificity for LRH-1.
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|---|---|
| ln Vitro |
In vitro, DLPC activates LRH-1. It is used as a phospholipid for biological studies.
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| ln Vivo |
In vivo, DLPC treatment induces bile acid biosynthetic enzymes in mouse liver, increases bile acid levels, and lowers hepatic triglycerides and serum glucose. DLPC treatment also decreases hepatic steatosis and improves glucose homeostasis in two mouse models of insulin resistance. It exhibits antidiabetic effects.
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| Enzyme Assay |
LRH-1 ligand binding assays are performed using cell-based reporter systems. Cells are transfected with LRH-1 expression plasmid and a luciferase reporter gene containing LRH-1 response elements. Cells are treated with DLPC, and luciferase activity is measured to quantify receptor activation. Competition binding assays may also be used to determine binding affinity.
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| Cell Assay |
Cellular assays for DLPC involve treating hepatocyte cell lines (e.g., HepG2) with the compound and measuring the expression of LRH-1 target genes (e.g., CYP7A1, SHP) by qPCR. Bile acid production and triglyceride levels in cell culture media are also quantified. DLPC is soluble in ethanol (99 mg/mL) and DMSO (7.14 mg/mL).
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| Animal Protocol |
In vivo animal studies typically involve oral or intraperitoneal administration of DLPC to mice. Liver tissue is collected for analysis of bile acid biosynthetic enzyme expression by qPCR and Western blot. Serum samples are analyzed for bile acid levels, triglycerides, and glucose.
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| ADME/Pharmacokinetics |
As a phospholipid, DLPC does not have conventional pharmacokinetic properties. When administered in vivo, the compound is incorporated into lipid metabolism pathways. Pharmacokinetic properties depend on the formulation and route of administration.
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| Toxicity/Toxicokinetics |
DLPC is a naturally occurring phospholipid and is generally considered biocompatible and low in toxicity. At research doses, no significant toxicity has been reported. Standard safety precautions for handling lipids should be followed. Storage conditions are powder at -20°C for up to 3 years.
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| References | |
| Additional Infomation |
1,2-Dilauroyl-sn-glycerol-3-phosphocholine is a phosphatidylcholine 24:0, wherein the acyl groups at positions 1 and 2 are designated as lauroyl (dodecanoyl). It is an LRH-1 agonist. It is a phosphatidylcholine 24:0 and also a dodecanoate ester. It is the conjugate base of 1,2-dilauroyl-sn-glycerol-3-phosphocholine (1+).
DLPC is a phospholipid used in the preparation and synthesis of liposomes. As an LRH-1 agonist, DLPC is a valuable tool for studying bile acid metabolism, liver function, and metabolic disorders including dyslipidemia and diabetes. It is used in spin-label and lipid studies. The purity is typically >98%. |
| Molecular Formula |
C32H64NO8P
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|---|---|
| Molecular Weight |
621.83
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| Exact Mass |
621.436
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| CAS # |
18194-25-7
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| Related CAS # |
(S)-1,2-Dilauroyl-sn-glycero-3-phosphocholine;127641-86-5
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| PubChem CID |
512874
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| Appearance |
White to off-white solid powder
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| LogP |
6.63
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
32
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| Heavy Atom Count |
42
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| Complexity |
705
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| Defined Atom Stereocenter Count |
1
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| SMILES |
CCCCCCCCCCCC(=O)OC[C@H](COP(=O)([O-])OCC[N+](C)(C)C)OC(=O)CCCCCCCCCCC
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| InChi Key |
IJFVSSZAOYLHEE-SSEXGKCCSA-N
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| InChi Code |
InChI=1S/C32H64NO8P/c1-6-8-10-12-14-16-18-20-22-24-31(34)38-28-30(29-40-42(36,37)39-27-26-33(3,4)5)41-32(35)25-23-21-19-17-15-13-11-9-7-2/h30H,6-29H2,1-5H3/t30-/m1/s1
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| Chemical Name |
[(2R)-2,3-di(dodecanoyloxy)propyl] 2-(trimethylazaniumyl)ethyl phosphate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
Ethanol: 100 mg/mL (160.82 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (4.02 mM) (saturation unknown) in 10% EtOH + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear EtOH stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (4.02 mM) (saturation unknown) in 10% EtOH + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear EtOH stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.6082 mL | 8.0408 mL | 16.0816 mL | |
| 5 mM | 0.3216 mL | 1.6082 mL | 3.2163 mL | |
| 10 mM | 0.1608 mL | 0.8041 mL | 1.6082 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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