| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
|
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| 100mg |
|
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| Other Sizes |
| Targets |
IC50: K channel[2]
|
|---|---|
| ln Vitro |
Blood-brain tumor barrier (BTB) permeability in RBMEC monolayers is significantly reduced by minoxidil suLfate (1-100 μM; 2 hours) in a time-dependent manner. Additionally, high concentrations of MS have a high effect on transendothelial electrical resistance (TEER) after 2 hours[2]. In brain tumor tissue, minoxidil sulfate (0-100 μM; 0-4 hours) elevated RhoA activity for 1 hour, peaking at 2 hours. Moreover, it strongly increased PKB phosphorylation at Ser-473 in a transitory manner without changing PKB levels overall. Furthermore, LY294002 partially inhibits PKB phosphorylation produced by MS [2].
|
| Cell Assay |
Western Blot Analysis[2]
Cell Types: Rat brain microvascular endothelial cells (RBMECs) Tested Concentrations: 0-100 μM Incubation Duration: 15 mins, 30 mins, 1 hour, 2 hrs (hours), 4 hrs (hours) Experimental Results: Induced RhoA activation and PKB phosphorylation at Ser -473. |
| References |
|
| Additional Infomation |
Minoxidil metabolites; structures are described in the first reference.
|
| Molecular Formula |
C9H15N5O4S
|
|---|---|
| Molecular Weight |
289.31
|
| Exact Mass |
289.084
|
| CAS # |
83701-22-8
|
| Related CAS # |
38304-91-5; 69935-18-8; 83701-22-8; 1020718-66-4
|
| PubChem CID |
138113345
|
| Appearance |
White to off-white solid powder
|
| Boiling Point |
732ºC at 760 mmHg
|
| Melting Point |
175-180°C
|
| Flash Point |
396.5ºC
|
| LogP |
0.72
|
| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
7
|
| Rotatable Bond Count |
3
|
| Heavy Atom Count |
19
|
| Complexity |
529
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
C1CCN(CC1)C2=CC(=N)N(C(=N2)N)OS(=O)(=O)O
|
| InChi Key |
KVOZMWUBYWDGEX-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C9H15N5O4S/c10-7-6-8(13-4-2-1-3-5-13)12-9(11)14(7)18-19(15,16)17/h6,10H,1-5H2,(H2,11,12)(H,15,16,17)
|
| Chemical Name |
(2-amino-6-imino-4-piperidin-1-ylpyrimidin-1-yl) hydrogen sulfate
|
| Synonyms |
U-10858; U10858 sulfate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: 250 mg/mL (864.12 mM)
H2O: 50 mg/mL (172.82 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (7.19 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (7.19 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (7.19 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.4565 mL | 17.2825 mL | 34.5650 mL | |
| 5 mM | 0.6913 mL | 3.4565 mL | 6.9130 mL | |
| 10 mM | 0.3457 mL | 1.7282 mL | 3.4565 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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