| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
Brassicin targets reactive oxygen species (ROS) and free radicals. It acts as a free radical scavenger, neutralizing peroxynitrite and DPPH radicals. The compound's antioxidant mechanism involves hydrogen atom transfer or electron donation to stabilize reactive species, thereby preventing oxidative damage to cellular components.
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|---|---|
| ln Vitro |
In vitro, Brassicin exhibits potent peroxynitrite and DPPH scavenging activities with IC50 values of 2.07 ± 0.17 µM and 13.3 µM, respectively. These activities demonstrate its strong antioxidant capacity. The compound's radical scavenging efficacy is comparable to or exceeds that of many standard antioxidants.
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| ln Vivo |
In vivo studies on Brassicin are limited in the available literature. As a flavonoid glycoside with antioxidant activity, it is expected to exert protective effects against oxidative stress-related conditions in animal models. Further in vivo investigations are needed to confirm its bioavailability and pharmacological efficacy in whole organisms.
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| Enzyme Assay |
Cell-free antioxidant assays for Brassicin include the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay, where the compound's ability to reduce the DPPH radical is measured spectrophotometrically at 517 nm. Peroxynitrite scavenging activity is assessed using a fluorescence-based assay with a peroxynitrite-sensitive probe. IC50 values are calculated from dose-response curves.
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| Cell Assay |
In vitro cellular assays for Brassicin involve treating cultured cells with the compound and measuring intracellular ROS levels using fluorescent probes such as DCFH-DA. Cells are typically exposed to oxidative stress inducers (e.g., H2O2 or peroxynitrite) in the presence or absence of Brassicin, and the protective effect is quantified by fluorescence intensity measurements.
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| Animal Protocol |
In vivo animal studies for Brassicin would typically involve oral or intraperitoneal administration in rodent models of oxidative stress or inflammation. Endpoints include measurements of serum and tissue antioxidant enzyme activities, lipid peroxidation markers, and histopathological examination of target organs. Dose-response relationships and bioavailability would be assessed.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of Brassicin are expected to be similar to those of other flavonoid glycosides. Oral bioavailability is generally low due to poor intestinal absorption and extensive first-pass metabolism. The compound may be deglycosylated by intestinal enzymes or gut microbiota to release the aglycone isorhamnetin, which is then absorbed and further metabolized.
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| Toxicity/Toxicokinetics |
Available toxicological data for Brassicin are limited. As a naturally occurring flavonoid found in dietary sources, it is generally considered safe at typical intake levels. No significant toxicity has been reported in standard assays. High-dose toxicity studies would be required for therapeutic development, but the compound's natural occurrence suggests a favorable safety profile.
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| References | |
| Additional Infomation |
Isorhamnetin 7-O-β-D-glucopyranoside is a glycosyloxyflavonoid formed by the substitution of isorhamnetin at the 7-position with a β-D-glucose group. It is a metabolite. It is a β-D-glucose glycoside, glycosyloxyflavonoid, monomethoxyflavonoid, monosaccharide derivative, and trihydroxyflavonoid. Functionally, it is related to both β-D-glucose and isorhamnetin. Isorhamnetin 7-glucopyranoside has been reported to be found in Zanthoxylum bungeanum, Sedum aizoon, and other organisms with relevant data.
Brassicin is a research compound with no clinical approvals. Its primary value lies in its antioxidant properties and potential applications in preventing oxidative stress-related diseases. The compound serves as a useful tool for studying free radical scavenging mechanisms and the biological activities of flavonoids. Its natural abundance makes it accessible for research purposes. |
| Molecular Formula |
C22H22O12
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|---|---|
| Molecular Weight |
478.40
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| Exact Mass |
478.111
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| CAS # |
6743-96-0
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| PubChem CID |
6455477
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| Appearance |
White to yellow solid powder
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| Boiling Point |
822.1±65.0 °C(Predicted)
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| LogP |
0.7
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| Hydrogen Bond Donor Count |
7
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| Hydrogen Bond Acceptor Count |
12
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
34
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| Complexity |
773
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| Defined Atom Stereocenter Count |
5
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| SMILES |
COC1=C(C=CC(=C1)C2=C(C(=O)C3=C(C=C(C=C3O2)O[C@H]4[C@@H]([C@H]([C@@H]([C@H](O4)CO)O)O)O)O)O)O
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| InChi Key |
YCUNOXSUHVGZRI-XMHBHJPISA-N
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| InChi Code |
InChI=1S/C22H22O12/c1-31-12-4-8(2-3-10(12)24)21-19(29)17(27)15-11(25)5-9(6-13(15)33-21)32-22-20(30)18(28)16(26)14(7-23)34-22/h2-6,14,16,18,20,22-26,28-30H,7H2,1H3/t14-,16-,18+,20-,22-/m1/s1
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| Chemical Name |
3,5-dihydroxy-2-(4-hydroxy-3-methoxyphenyl)-7-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxychromen-4-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 66.67 mg/mL (139.36 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (5.23 mM) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.0903 mL | 10.4515 mL | 20.9030 mL | |
| 5 mM | 0.4181 mL | 2.0903 mL | 4.1806 mL | |
| 10 mM | 0.2090 mL | 1.0452 mL | 2.0903 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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