| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
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| ln Vitro |
The cell viability of rat primary chondrocytes and human osteoarthritis chondrocytes is not affected by BNTA (0.01-10 μM; 1-7 d)[1]. SOX9 protein is significantly increased by BNTA (0.1 μM; 2 d)[1]. BNTA (0.1 μM; 2 d) significantly raises the levels of SOX9 and COL2A1 proteins in rat OA chondrocytes generated by IL1β[1]. In ATDC5 cells, BNTA (10 μM; 5 d) enhances proteoglycan staining[1]. In human OA chondrocytes, BNTA (0.01-10 μM; 6 h) increases the expression levels of ECM-related genes COL2A1, ACAN, proteoglycan 4 (PRG4), and SRY-box 9 (SOX9)[1]. In IL1β-induced rat OA chondrocytes, BNTA (0.01-10 μM; 6 h) raises Col2a1, Acan, Prg4, and Sox9 mRNA levels; the effects peak at 0.1 μM[1]. In arthritic cartilage explants, BNTA (0.01-1 μM; 2 or 3 w) increases anabolism and reduces inflammatory response[1].
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| ln Vivo |
In rats undergoing anterior cruciate ligament transection (ACLT), BNTA (0.015–1.5 mg/kg; intra-articular injection; twice weekly for 4 and 8 weeks) was able to slow the progression of osteoarthritis (OA)[1].
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| Cell Assay |
Cell Viability Assay[1]
Cell Types: Human OA chondrocytes Tested Concentrations: 0.01, 0.1, 1, 10 μM Incubation Duration: 1, 3, 5, 7 d Experimental Results: No toxicity was observed. Western Blot Analysis[1] Cell Types: Human OA chondrocytes Tested Concentrations: 0.1 μM Incubation Duration: 2 d Experimental Results: Elevated SOX9 protein compared with vehicle. |
| Animal Protocol |
Animal/Disease Models: Male SD rats weighing 80 g are induced by ACLT[1]
Doses: 0.015, 0.15, 1.5 mg/kg Route of Administration: Intra-articular injection; twice a week for 4 and 8 weeks Experimental Results: Attenuated post-traumatic osteoarthritis development after intra-articular injection for 4 and 8 weeks and was well tolerated. |
| References |
[1]. Shi Y, et, al. A small molecule promotes cartilage extracellular matrix generation and inhibits osteoarthritis development. Nat Commun. 2019 Apr 23; 10(1): 1914.
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| Molecular Formula |
C17H11BRCLNO3S2
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|---|---|
| Molecular Weight |
456.76
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| CAS # |
685119-25-9
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| SMILES |
BrC1=C(C(=C([H])S1)S(C1C([H])=C([H])C([H])=C([H])C=1[H])(=O)=O)N([H])C(C1=C([H])C([H])=C([H])C([H])=C1Cl)=O
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 100 mg/mL (218.93 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.47 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (5.47 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1893 mL | 10.9467 mL | 21.8933 mL | |
| 5 mM | 0.4379 mL | 2.1893 mL | 4.3787 mL | |
| 10 mM | 0.2189 mL | 1.0947 mL | 2.1893 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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