| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
Reactive Oxygen Species (ROS); Glutathione (GSH) synthesis pathway; Hydrogen sulfide (H2S) production. NACET acts by replenishing intracellular GSH stores, thereby scavenging ROS and protecting cells from oxidative stress. It also serves as a donor of H2S, which exerts anti-inflammatory and cytoprotective effects. Additionally, it has been shown to exhibit diverse effects including inhibition of protein synthesis, DNA replication, and induction of apoptosis.
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| ln Vitro |
N-acetyl-L-cysteine ethyl ester is N-acetyl-L-cysteine (NAC) in an esterified form. N-Acetyl-L-cysteine ethyl ester produces cysteine and NAC and shows increased cell permeability. N-Acetyl-L-cysteine ethyl ester can be employed as an H2S producer and raises the amount of hydrogen sulfide (H2S) in the air. As a mucolytic agent and GSH-related antioxidant, N-Acetyl-L-cysteine ethyl ester may replace NAC[1].
In vitro, NACET (1 mM) increases glutathione levels in various cell types. It protects oxidative-stressed retinal pigment epithelial (RPE) cells by increasing viability. It acts as an H2S producer. In cell-free systems, it directly scavenges ROS via its thiol group. It also reduces increases in plasma AST, ALT, and LDH levels induced by paracetamol in rat models, although these data are derived from in vivo studies. It is a potent antioxidant that prevents oxidative cellular damage. |
| ln Vivo |
In vivo, NACET (50 mg/kg twice per day for two weeks) increases glutathione levels in rat liver, kidney, heart, testis, and brain. It reduces increases in plasma AST, ALT, and LDH induced by paracetamol (acetaminophen) overdose in rats, demonstrating hepatoprotective effects. It also increases circulating H2S levels. These findings support its use in models of oxidative stress, such as acetaminophen toxicity, neurodegeneration, and ischemia-reperfusion injury.
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| Enzyme Assay |
For ROS scavenging assays, prepare NACET solutions in PBS or buffer. For DPPH radical scavenging: mix NACET (0-1000 uM) with DPPH (0.1 mM in methanol). Incubate in dark for 30 min at room temperature. Measure absorbance at 517 nm. Calculate % scavenging. For ABTS assay: generate ABTS radical cation by reacting ABTS with potassium persulfate. Add NACET and measure decolorization at 734 nm. These cell-free assays quantify direct antioxidant capacity.
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| Cell Assay |
For cell viability and GSH assays, seed cells (e.g., RPE, hepatocytes) in 96-well plates. Treat with NACET (0.1-10 mM) for 24-48 hours. For oxidative stress challenge, co-treat with H2O2 or acetaminophen. Measure cell viability via MTT or CCK-8. Measure intracellular GSH using a GSH-Glo or DTNB-based assay. For ROS measurement, use DCFH-DA probe and measure fluorescence at 485/535 nm. NACET should increase GSH and reduce ROS levels.
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| Animal Protocol |
For acetaminophen-induced hepatotoxicity model: Male rats (200-250 g) are fasted overnight. Administer acetaminophen (1-3 g/kg, oral) to induce liver injury. NACET is administered (e.g., 50-200 mg/kg, intraperitoneal or oral) 1-2 hours before or after acetaminophen. Collect blood at 4-24 hours for serum ALT, AST, and LDH assays. Harvest liver tissue for histopathology (H&E staining) and GSH measurement. Monitor body weight and behavior. Compare efficacy to standard NAC treatment.
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| ADME/Pharmacokinetics |
NACET (MW 207.25; C₇H13NO3S). Appearance: White to off-white crystalline powder. Solubility: Soluble in water (≥10 mg/mL) and organic solvents (DMSO, ethanol). Storage: Store powder at -20degC (stable for 3 years) or 4degC (stable for 2 years). In solution at -80degC (stable for 6 months). Protect from light and moisture. Solutions are stable at room temperature for up to 24 hours. It has improved lipophilicity over NAC (LogP value not publicly listed).
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| Toxicity/Toxicokinetics |
NACET is considered safe at typical research doses (50-200 mg/kg in rodents). It is an ethyl ester, which may hydrolyze in the stomach to NAC and ethanol; however, the ethanol produced is negligible. High doses may cause gastrointestinal distress. As it increases H2S, very high doses might cause hypotension. Always follow standard laboratory safety guidelines. Wear gloves and safety glasses. Not approved for human therapeutic use, though NAC is an FDA-approved drug. Consult SDS.
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| References | |
| Additional Infomation |
NACET is a more lipophilic alternative to NAC (N-acetylcysteine), offering better oral absorption and cellular penetration. It is being studied for various applications including psychiatric disorders (as a glutamate modulator), chronic obstructive pulmonary disease (COPD), and contrast-induced nephropathy. Unlike NAC, NACET is an effective H2S donor, which may contribute to its anti-inflammatory effects. It is a research-use-only compound and not currently approved by the FDA for clinical use, though it is widely studied as a nutraceutical.
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| Molecular Formula |
C7H13NO3S
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|---|---|
| Molecular Weight |
191.25
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| Exact Mass |
191.062
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| CAS # |
59587-09-6
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| PubChem CID |
148861
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| Appearance |
White to off-white solid powder
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| Density |
1.138g/cm3
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| Boiling Point |
337.6ºC at 760 mmHg
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| Flash Point |
158ºC
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| Index of Refraction |
1.48
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| LogP |
0.824
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
12
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| Complexity |
172
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| Defined Atom Stereocenter Count |
1
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| SMILES |
CCOC(=O)[C@H](CS)NC(=O)C
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| InChi Key |
MSMRAGNKRYVTCX-LURJTMIESA-N
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| InChi Code |
InChI=1S/C7H13NO3S/c1-3-11-7(10)6(4-12)8-5(2)9/h6,12H,3-4H2,1-2H3,(H,8,9)/t6-/m0/s1
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| Chemical Name |
ethyl (2R)-2-acetamido-3-sulfanylpropanoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: ≥ 100 mg/mL (522.88 mM)
DMSO: 50 mg/mL (261.44 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (13.07 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. Solubility in Formulation 2: ≥ 1 mg/mL (5.23 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 10.0 mg/mL clear DMSO stock solution to 400 μL of PEG300 and mix evenly; then add 50 μL of Tween-80 to the above solution and mix evenly; then add 450 μL of normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 1 mg/mL (5.23 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.2288 mL | 26.1438 mL | 52.2876 mL | |
| 5 mM | 1.0458 mL | 5.2288 mL | 10.4575 mL | |
| 10 mM | 0.5229 mL | 2.6144 mL | 5.2288 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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