| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
|
||
| 10mg |
|
||
| 50mg |
|
||
| 100mg |
|
||
| Other Sizes |
| Targets |
Human Endogenous Metabolite
D-Ribose 5-phosphate disodium dihydrate targets the pentose phosphate pathway (PPP). As a key intermediate, it is used in the synthesis of nucleotides and nucleic acids. It is involved in ATP biosynthesis through the oxidative phase of the PPP. It is also a substrate for phosphoribosyltransferases. |
|---|---|
| ln Vitro |
Most people believe that the oxidative and nonoxidative pentose phosphate pathways produce ribose-5-phosphate[1].
In vitro, D-Ribose 5-phosphate disodium dihydrate is used as a substrate in enzymatic assays for enzymes of the pentose phosphate pathway, including glucose-6-phosphate dehydrogenase and transketolase. It supports investigations into energy metabolism, oxidative stress responses, and enzymatic reactions involving phosphoribosyltransferases. |
| ln Vivo |
In vivo, D-Ribose 5-phosphate disodium dihydrate is an intermediate of the pentose phosphate pathway. It participates in nucleotide and nucleic acid synthesis and ATP biosynthesis. It can be used to study diseases caused by riboflavin deficiency and endocrine and metabolic diseases.
|
| Enzyme Assay |
In vitro enzyme assays for pentose phosphate pathway enzymes involve incubating the enzyme with D-Ribose 5-phosphate disodium dihydrate as substrate in buffer at pH 7.4-8.0. For transketolase assays, the production of sedoheptulose-7-phosphate or glyceraldehyde-3-phosphate is measured spectrophotometrically or by HPLC. For phosphoribosyltransferase assays, the formation of nucleotide monophosphates is quantified.
|
| Cell Assay |
For in vitro cell assays, cells are cultured and treated with D-Ribose 5-phosphate disodium dihydrate at concentrations ranging from 0.1-10 mM. Nucleotide levels are analyzed by LC-MS/MS. Pentose phosphate pathway flux is assessed by measuring the production of NADPH and ribose-5-phosphate. Oxidative stress responses are evaluated by measuring ROS levels and glutathione content.
|
| Animal Protocol |
For in vivo animal studies, D-Ribose 5-phosphate disodium dihydrate is typically administered to rodents via oral gavage or intravenous injection in metabolic studies. Blood and tissue samples are collected, and nucleotide and metabolite levels are quantified by LC-MS/MS. The compound's effects on the pentose phosphate pathway and energy metabolism are assessed.
|
| ADME/Pharmacokinetics |
D-Ribose 5-phosphate disodium dihydrate (MW ~350.09) has the molecular formula C5H9Na2O8P·2H2O. It is a disodium salt dihydrate form that is highly soluble in water. The compound is stable under recommended storage conditions. It is an intermediate of the pentose phosphate pathway and a precursor of nucleotides and nucleic acids.
|
| Toxicity/Toxicokinetics |
The compound is for research use only. No specific toxicity data are available. As an endogenous metabolite, it is expected to have low toxicity at physiological concentrations. Standard laboratory safety precautions should be followed when handling this compound.
|
| References | |
| Additional Infomation |
D-Ribose 5-phosphate disodium dihydrate (CAS# 207671-46-3) is a key intermediate of the pentose phosphate pathway and a precursor of nucleotides and nucleic acids. It has not been approved as a therapeutic drug. The compound is widely used in biochemical and metabolic research to study energy metabolism, oxidative stress, and nucleotide biosynthesis. It is also used in studies of riboflavin deficiency and endocrine/metabolic diseases.
|
| Molecular Formula |
C5H13NA2O10P
|
|---|---|
| Molecular Weight |
310.10
|
| Exact Mass |
273.983
|
| CAS # |
207671-46-3
|
| Related CAS # |
D-Ribose 5-phosphate disodium;18265-46-8;D-Ribose 5-phosphate;4300-28-1
|
| PubChem CID |
71310163
|
| Appearance |
White to yellow solid powder
|
| Hydrogen Bond Donor Count |
5
|
| Hydrogen Bond Acceptor Count |
10
|
| Rotatable Bond Count |
5
|
| Heavy Atom Count |
18
|
| Complexity |
212
|
| Defined Atom Stereocenter Count |
3
|
| SMILES |
C([C@H]([C@H]([C@H](C=O)O)O)O)OP(=O)([O-])[O-].O.O.[Na+].[Na+]
|
| InChi Key |
ROFJYRUKQPRHKK-WFAHMUOKSA-L
|
| InChi Code |
InChI=1S/C5H11O8P.2Na.2H2O/c6-1-3(7)5(9)4(8)2-13-14(10,11)12;;;;/h1,3-5,7-9H,2H2,(H2,10,11,12);;;2*1H2/q;2*+1;;/p-2/t3-,4+,5-;;;;/m0..../s1
|
| Chemical Name |
disodium;[(2R,3R,4R)-2,3,4-trihydroxy-5-oxopentyl] phosphate;dihydrate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O: 100 mg/mL (322.48 mM)
DMSO: 1 mg/mL (3.22 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 50 mg/mL (161.24 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.2248 mL | 16.1238 mL | 32.2477 mL | |
| 5 mM | 0.6450 mL | 3.2248 mL | 6.4495 mL | |
| 10 mM | 0.3225 mL | 1.6124 mL | 3.2248 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
