| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
Human Endogenous Metabolite
D-Ribose 5-phosphate is a central metabolite in the pentose phosphate pathway, which is responsible for the generation of NADPH and pentose sugars. The compound serves as a substrate for enzymes such as ribose-5-phosphate isomerase, which converts it to ribulose-5-phosphate, and phosphoribosyl pyrophosphate synthetase, which converts it to PRPP for nucleotide biosynthesis. Its primary targets are the enzymes of the pentose phosphate pathway and nucleotide synthesis pathways. As a biochemical reagent, it is used to study these metabolic pathways and enzyme activities. |
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| ln Vitro |
It is commonly accepted that both oxidative and non-oxidative pentose phosphate pathways can create ribose-5-phosphate [1].
In vitro studies demonstrate that D-Ribose 5-phosphate disodium is a substrate for various enzymes involved in the pentose phosphate pathway and nucleotide biosynthesis. It is used in enzyme assays to measure the activity of ribose-5-phosphate isomerase, phosphoribosyl pyrophosphate synthetase, and other related enzymes. The compound's activity is assessed by measuring the formation of reaction products using spectrophotometric, chromatographic, or enzymatic coupled assays. Its stability in solution is typically confirmed by HPLC or NMR analysis. The compound is also used in cell culture media to support nucleotide synthesis and cellular metabolism. |
| ln Vivo |
In vivo, D-Ribose 5-phosphate disodium is a naturally occurring metabolite that plays a critical role in cellular metabolism. It is generated in cells through the pentose phosphate pathway and is utilized for nucleotide synthesis, which is essential for DNA replication, RNA transcription, and cellular proliferation. The compound's in vivo levels are regulated by the balance between its production through the PPP and its consumption in nucleotide synthesis. As a research reagent, it may be administered to animal models to study metabolic fluxes, though its rapid metabolism limits its utility as a therapeutic agent.
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| Enzyme Assay |
In vitro enzyme/receptor binding assays for D-Ribose 5-phosphate disodium involve measuring its utilization as a substrate by enzymes of the pentose phosphate pathway and nucleotide synthesis. The assay typically uses purified enzymes such as ribose-5-phosphate isomerase, phosphoribosyl pyrophosphate synthetase, or transketolase. The compound is incubated with the enzyme and appropriate cofactors (e.g., ATP for PRPP synthetase), and product formation is quantified spectrophotometrically or by HPLC. Kinetic parameters (Km, Vmax) are determined from substrate concentration-response curves. The compound's stability and purity are confirmed by HPLC or NMR analysis prior to use.
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| Cell Assay |
Cellular assays for D-Ribose 5-phosphate disodium are conducted to study its role in cellular metabolism and nucleotide synthesis. Cells are cultured in media containing the compound, and its effects on nucleotide pools, metabolic flux, and cell proliferation are assessed. Metabolic flux analysis using isotopically labeled ribose-5-phosphate can be performed to trace its incorporation into nucleotides and other metabolites. The compound's effects on cell viability and proliferation are assessed using MTT or cell counting assays. Its role in supporting cellular metabolism under conditions of oxidative stress or nutrient deprivation may also be investigated.
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| Animal Protocol |
In vivo animal studies with D-Ribose 5-phosphate disodium are limited, as the compound is a naturally occurring metabolite rather than a therapeutic agent. When administered to animals, the compound is rapidly metabolized and incorporated into cellular pathways. It may be used in metabolic flux studies where isotopically labeled ribose-5-phosphate is administered to trace the flow of carbon through the pentose phosphate pathway and nucleotide synthesis. Tissue samples are collected and analyzed by mass spectrometry to determine the incorporation of labeled carbon into nucleotides and other metabolites. The compound's effects on metabolic pathways in disease models may also be investigated.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of D-Ribose 5-phosphate disodium are characteristic of phosphorylated sugars. The compound has a molecular weight of 274.07 g/mol and a molecular formula of C₅H₉Na2O₈P. The disodium salt form enhances aqueous solubility. As a charged molecule, the compound is membrane-impermeant and requires transporters for cellular uptake. Following administration, it is rapidly metabolized by cellular enzymes and incorporated into metabolic pathways. Its half-life in circulation is expected to be short due to rapid metabolism and clearance. The compound is typically stored at -20degC and is soluble in water at 50 mg/mL.
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| Toxicity/Toxicokinetics |
Toxicological data for D-Ribose 5-phosphate disodium are limited, as the compound is a naturally occurring metabolite rather than a therapeutic agent. The compound is not intended for human therapeutic use and is supplied for research purposes only. As an endogenous metabolite, it is generally considered to have low toxicity at physiological concentrations. In cell-based assays, the compound is well-tolerated at concentrations used for metabolic studies (typically 1-10 mM). High concentrations may cause osmotic effects or metabolic imbalances. Standard safety precautions should be followed when handling this compound.
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| References | |
| Additional Infomation |
See also: d-ribose, 5-(dihydrophosphate), disodium salt (note moved to).
D-Ribose 5-phosphate disodium is a key intermediate in the pentose phosphate pathway and a precursor for nucleotide and nucleic acid synthesis. It is used as a biochemical reagent in the synthesis of nucleotides and nucleic acids. The compound is an intermediate of the oxidative branch of the PPP and the final product of the non-oxidative branch of PPP. It is a naturally occurring metabolite with no therapeutic indications. The compound is available in high purity (≥85%) and is typically stored at -20degC. |
| Molecular Formula |
C5H9NA2O8P
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|---|---|
| Molecular Weight |
274.07
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| Exact Mass |
273.983
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| CAS # |
18265-46-8
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| Related CAS # |
D-Ribose 5-phosphate disodium dihydrate;207671-46-3;D-Ribose 5-phosphate;4300-28-1
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| PubChem CID |
18646925
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| Appearance |
White to off-white solid powder
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| Boiling Point |
539.9ºC at 760mmHg
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| Flash Point |
280.3ºC
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| Vapour Pressure |
6.58E-14mmHg at 25°C
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
16
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| Complexity |
212
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| Defined Atom Stereocenter Count |
3
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| SMILES |
C([C@H]([C@H]([C@H](C=O)O)O)O)OP(=O)([O-])[O-].[Na+].[Na+]
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| InChi Key |
MSUSOPCULOEKKB-LMQMBFIUSA-L
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| InChi Code |
InChI=1S/C5H11O8P.2Na/c6-1-3(7)5(9)4(8)2-13-14(10,11)12;;/h1,3-5,7-9H,2H2,(H2,10,11,12);;/q;2*+1/p-2/t3-,4+,5-;;/m0../s1
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| Chemical Name |
disodium;[(2R,3R,4R)-2,3,4-trihydroxy-5-oxopentyl] phosphate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : 83.33 mg/mL (304.05 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (364.87 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.6487 mL | 18.2435 mL | 36.4870 mL | |
| 5 mM | 0.7297 mL | 3.6487 mL | 7.2974 mL | |
| 10 mM | 0.3649 mL | 1.8244 mL | 3.6487 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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