| Size | Price | Stock | Qty |
|---|---|---|---|
| 10g |
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| 25g |
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| 50g | |||
| Other Sizes |
| Targets |
1-Aminohydantoin hydrochloride exhibits various biological activities, including anticonvulsant, antitumor, and antiviral activities. The mechanism of action involves the inhibition of enzymes and receptors involved in cell function and signal transduction. It also has potential antimicrobial and antitumor properties.
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|---|---|
| ln Vitro |
In vitro, 1-Aminohydantoin hydrochloride exhibits various biological activities, including antimicrobial, antitumor, anticonvulsant, and antiviral properties. It is used as a standard for the determination of residues of veterinary agents in meat, milk, and other products.
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| ln Vivo |
In vivo, 1-Aminohydantoin hydrochloride is a major metabolite of nitrofurantoin in animal tissues. It binds covalently to tissue proteins and is released under slightly acidic conditions. It can be detected by derivatization with 2-nitrobenzaldehyde forming a nitrophenyl derivative of AHD.
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| Enzyme Assay |
In vitro assays for 1-Aminohydantoin hydrochloride typically involve its detection and quantification as a metabolite of nitrofurantoin. The compound is extracted from tissues and detected by derivatization with 2-nitrobenzaldehyde, forming a nitrophenyl derivative that can be analyzed by HPLC or LC-MS. Enzyme inhibition assays are used to study its mechanism of action.
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| Cell Assay |
For in vitro cell assays, cells are cultured and treated with 1-Aminohydantoin hydrochloride at various concentrations. Cell viability is assessed by MTT assay. Antitumor activity is evaluated by measuring cell proliferation inhibition. Antimicrobial activity is assessed by measuring bacterial growth inhibition. The compound's effects on enzyme activity and signal transduction are evaluated.
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| Animal Protocol |
For in vivo animal studies, 1-Aminohydantoin hydrochloride is studied as a metabolite of nitrofurantoin. Animals are administered nitrofurantoin, and tissue samples are collected. The compound is extracted and detected by derivatization with 2-nitrobenzaldehyde. Its covalent binding to tissue proteins is studied.
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| ADME/Pharmacokinetics |
1-Aminohydantoin hydrochloride (MW 151.55) has the molecular formula C₃H₆ClN₃O₂. It is a white to off-white solid. The compound is stable under recommended storage conditions. It is soluble in water and organic solvents. It binds covalently to tissue proteins.
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| Toxicity/Toxicokinetics |
The compound is for research use only. No specific toxicity data are available. As a metabolite of nitrofurantoin, its toxicity profile is expected to be related to the parent drug. Standard laboratory safety precautions should be followed when handling this compound.
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| References |
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| Additional Infomation |
1-Aminohydantoin hydrochloride (CAS# 2827-56-7) is a major metabolite of the antibiotic nitrofurantoin. It has not been approved as a therapeutic agent. The compound is used as a standard for the determination of veterinary drug residues in food products and in research on anticonvulsant, antitumor, and antiviral activities.
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| Molecular Formula |
C3H6CLN3O2
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|---|---|
| Molecular Weight |
151.55
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| Exact Mass |
151.014
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| CAS # |
2827-56-7
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| PubChem CID |
12472963
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| Appearance |
White to off-white solid powder
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| Boiling Point |
256.8ºC at 760 mmHg
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| Melting Point |
201-205 °C(lit.)
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| Flash Point |
109.1ºC
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| Vapour Pressure |
0.0119mmHg at 25°C
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| LogP |
0.18
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
9
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| Complexity |
144
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1C(=O)NC(=O)N1N.Cl
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| InChi Key |
WEOHANUVLKERQI-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C3H5N3O2.ClH/c4-6-1-2(7)5-3(6)8;/h1,4H2,(H,5,7,8);1H
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| Chemical Name |
1-aminoimidazolidine-2,4-dione;hydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 25 mg/mL (164.96 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (16.50 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (16.50 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (16.50 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.5985 mL | 32.9924 mL | 65.9848 mL | |
| 5 mM | 1.3197 mL | 6.5985 mL | 13.1970 mL | |
| 10 mM | 0.6598 mL | 3.2992 mL | 6.5985 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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