| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg | |||
| Other Sizes |
| Targets |
Ferulic acid acyl-beta-D-glucoside has no direct pharmacological target of its own. The parent compound, ferulic acid, is a known inhibitor of the fibroblast growth factor receptor 1 (FGFR1) with IC50 values of 3.78 and 12.5 microM for FGFR1 and FGFR2, respectively. It also exhibits potent antioxidant activity. The glucoside conjugate is generally considered a storage or transport form of ferulic acid, which can be hydrolyzed to release the active parent compound in the body.
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|---|---|
| ln Vitro |
In vitro, ferulic acid acyl-beta-D-glucoside is typically used as a standard to study its own stability or its conversion to ferulic acid. The biological activity of ferulic acid itself is well-documented and includes FGFR1 inhibition, antioxidant, anti-inflammatory, and anti-proliferative effects in various cancer cell lines. The acyl-beta-D-glucoside is generally less active than the parent aglycone but is a key intermediate in its metabolism.
|
| ln Vivo |
In vivo, ferulic acid acyl-beta-D-glucoside is a circulating metabolite that contributes to the bioavailability of ferulic acid. After consumption of ferulic acid-rich foods (e.g., coffee, grains, fruits), this glucoside is one of the forms found in the bloodstream. It may serve as a reservoir of ferulic acid, being hydrolyzed back to the active parent compound in the liver or kidneys by beta-glucosidase enzymes, thereby prolonging its biological effects.
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| Enzyme Assay |
Ferulic acid acyl-beta-D-glucoside is used as an analytical standard for non-cellular assays. For LC-MS or HPLC method development, a stock solution is prepared by dissolving the compound in an organic solvent like methanol or DMSO. This solution is used to generate a calibration curve to identify and quantify this metabolite in complex plant extracts or biological samples (e.g., plasma, urine). It ensures accurate tracking of this specific compound during research.
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| Cell Assay |
For in vitro cellular experiments, Ferulic acid acyl-beta-D-glucoside is used as an analytical standard and, in some cases, to study its hydrolysis. For example, Caco-2 intestinal cells are cultured and treated with ferulic acid acyl-beta-D-glucoside. The culture media is collected at different time points, and the standard is used to quantify the amount of glucoside that has been taken up by the cells. LC-MS can be used to measure both the parent glucoside and free ferulic acid, allowing researchers to determine the rate of cellular hydrolysis by endogenous glucosidases.
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| Animal Protocol |
For in vivo animal studies, Ferulic acid acyl-beta-D-glucoside is not administered as a drug but is used as an analytical standard. For example, in a rat study, animals are orally administered a polyphenol-rich extract containing ferulic acid. Blood plasma samples are collected over time. The glucoside standard is used to create a calibration curve for LC-MS to measure the concentration of this specific metabolite in the plasma. This data is used to determine the absorption and metabolism of dietary ferulic acid in the body, showing how it is conjugated to reach the bloodstream.
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| Toxicity/Toxicokinetics |
As a naturally occurring dietary metabolite, Ferulic acid acyl-beta-D-glucoside is considered to have low toxicity and is generally recognized as safe (GRAS) for research purposes. The compound is a normal component of the human diet. Standard laboratory safety precautions for handling organic compounds should be used. Not intended for human consumption.
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| References | |
| Additional Infomation |
1-O-feruloyl-β-D-glucose is a β-D-glucose glycoside formed by the condensation of the carboxyl group of ferulic acid with the terminal hydroxyl group of β-D-glucose. It possesses antioxidant activity and is also a plant metabolite. It is a β-D-glucose glycoside, cinnamic acid ester, phenolic compound, and aromatic ether. Its function is related to ferulic acid. 1-O-feruloyl-β-D-glucose has been reported to exist in tea plant (Camellia sinensis), privet (Ligustrum obtusifolium), and other organisms with relevant data.
Ferulic acid acyl-beta-D-glucoside is not a drug but a research-use analytical standard and dietary metabolite. It is used to study the metabolism, bioavailability, and bioactivity of ferulic acid, a major dietary polyphenol. Its applications are in the fields of nutritional science, pharmacognosy, and drug metabolism, helping to explain how the health benefits of a diet rich in whole grains and plants are manifested at the molecular level. |
| Molecular Formula |
C16H20O9
|
|---|---|
| Molecular Weight |
356.32
|
| Exact Mass |
356.111
|
| CAS # |
7196-71-6
|
| PubChem CID |
13962928
|
| Appearance |
White to off-white solid powder
|
| LogP |
-0.3
|
| Hydrogen Bond Donor Count |
5
|
| Hydrogen Bond Acceptor Count |
9
|
| Rotatable Bond Count |
6
|
| Heavy Atom Count |
25
|
| Complexity |
469
|
| Defined Atom Stereocenter Count |
5
|
| SMILES |
COC1=C(C=CC(=C1)/C=C/C(=O)O[C@H]2[C@@H]([C@H]([C@@H]([C@H](O2)CO)O)O)O)O
|
| InChi Key |
JWRQVQWBNRGGPK-PMQCXRHVSA-N
|
| InChi Code |
InChI=1S/C16H20O9/c1-23-10-6-8(2-4-9(10)18)3-5-12(19)25-16-15(22)14(21)13(20)11(7-17)24-16/h2-6,11,13-18,20-22H,7H2,1H3/b5-3+/t11-,13-,14+,15-,16+/m1/s1
|
| Chemical Name |
[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] (E)-3-(4-hydroxy-3-methoxyphenyl)prop-2-enoate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O: 50 mg/mL (140.32 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 25 mg/mL (70.16 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication (<60°C).
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.8065 mL | 14.0323 mL | 28.0647 mL | |
| 5 mM | 0.5613 mL | 2.8065 mL | 5.6129 mL | |
| 10 mM | 0.2806 mL | 1.4032 mL | 2.8065 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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