| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
3-Amino-2-oxazolidinone does not have a defined pharmacological target. It is a metabolite of furazolidone and serves as a biomarker for exposure to this banned antibiotic. Its detection in animal tissues indicates the prior administration of furazolidone.
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|---|---|
| ln Vitro |
In vitro, 3-amino-2-oxazolidinone is used as an analytical standard for the detection of nitrofuran metabolites in food products. It does not exhibit significant biological activity itself. Its primary use is in the development and validation of analytical methods for food safety testing.
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| ln Vivo |
In vivo, 3-amino-2-oxazolidinone is a metabolite that forms from the breakdown of furazolidone in animals. It can bind to tissue proteins, forming stable residues that persist for long periods. Its detection in edible tissues is a key indicator of illegal furazolidone use.
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| Enzyme Assay |
In vitro assays for 3-amino-2-oxazolidinone typically involve its extraction from food or tissue samples and analysis by LC-MS/MS. The compound is derivatized with 2-nitrobenzaldehyde to form a more detectable derivative. It is used as a reference standard for method development and validation in food safety laboratories.
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| Cell Assay |
For in vitro cell assays, 3-amino-2-oxazolidinone is not typically used as a treatment. It is used as a standard in analytical chemistry. Cell-based assays may be employed to study the toxicity or metabolic fate of nitrofurans, but AOZ itself is primarily a marker compound.
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| Animal Protocol |
For in vivo animal studies, 3-amino-2-oxazolidinone is studied as a marker of furazolidone exposure. Animals are administered furazolidone, and tissue samples are collected. The compound is extracted and quantified by LC-MS to determine residue levels and assess compliance with food safety regulations.
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| ADME/Pharmacokinetics |
3-Amino-2-oxazolidinone (MW 102.09) has the molecular formula C₃H₆N₂O₂. It is a solid compound. It is soluble in water and organic solvents. The compound is stable under recommended storage conditions. It is also known as AOZ.
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| Toxicity/Toxicokinetics |
The compound is for research use only. No specific toxicity data are available for 3-amino-2-oxazolidinone. Its significance lies in its role as a metabolite of a banned antibiotic. Standard laboratory safety precautions should be followed when handling this compound.
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| References |
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| Additional Infomation |
3-Amino-2-oxazolidinone is an oxazolidinone.
3-Amino-2-oxazolidinone (CAS# 80-65-9) is the major metabolite of the veterinary drug furazolidone. It has not been approved as a therapeutic agent. The compound is used as an analytical standard in food safety testing for the detection of banned nitrofuran antibiotics in animal-derived food products. It is also known as AOZ. |
| Molecular Formula |
C3H6N2O2
|
|---|---|
| Molecular Weight |
102.09
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| Exact Mass |
102.042
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| CAS # |
80-65-9
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| Related CAS # |
3-Amino-2-oxazolidinone-d4;1188331-23-8
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| PubChem CID |
65725
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| Appearance |
Colorless to off-white solid powder
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
167.2±23.0 °C at 760 mmHg
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| Melting Point |
65-67ºC
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| Flash Point |
54.9±22.6 °C
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| Vapour Pressure |
1.7±0.3 mmHg at 25°C
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| Index of Refraction |
1.518
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| LogP |
-1.75
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
0
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| Heavy Atom Count |
7
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| Complexity |
92.9
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| Defined Atom Stereocenter Count |
0
|
| SMILES |
C1COC(=O)N1N
|
| InChi Key |
KYCJNIUHWNJNCT-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C3H6N2O2/c4-5-1-2-7-3(5)6/h1-2,4H2
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| Chemical Name |
3-amino-1,3-oxazolidin-2-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 25 mg/mL (244.88 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (24.49 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (24.49 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (24.49 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 9.7953 mL | 48.9764 mL | 97.9528 mL | |
| 5 mM | 1.9591 mL | 9.7953 mL | 19.5906 mL | |
| 10 mM | 0.9795 mL | 4.8976 mL | 9.7953 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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