| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
|
||
| Other Sizes |
| Targets |
Ki: 32.6 μM (TPH)[1]
p-Ethynylphenylalanine hydrochloride targets tryptophan hydroxylase (TPH), the rate-limiting enzyme in serotonin biosynthesis. It is a potent, selective, reversible, and competitive inhibitor of TPH. p-Ethynylphenylalanine hydrochloride has a Ki of 32.6 μM. |
|---|---|
| ln Vitro |
Serotonin production is specifically and reversibly inhibited by p-phenylphenylalanine hydrochloride [1]. A range of recombinant 5-HT receptors, including 5-HT1, 5-HT2, 5-HT4, 5-HT5, 5-HT6, and 5-HT7, are impacted by p-Ethynylphenylalanine hydrochloride [1].
p-Ethynylphenylalanine hydrochloride demonstrates potent in vitro inhibition of tryptophan hydroxylase with a Ki of 32.6 μM. It is a competitive inhibitor of TPH. |
| ln Vivo |
In rat midbrain but tissue, p-Ethynylphenylalanine hydrochloride (30 mg/kg; ip) lowers 5-HT and 5-HIAA levels[1]. Arbuscular amino acid decarboxylase is not inhibited by p-Ethynylphenylalanine hydrochloride[1].
p-Ethynylphenylalanine hydrochloride has potential in vivo applications for selectively and reversibly inhibiting serotonin biosynthesis. This may contribute to characterizing the role of serotonin in behavioral and physiological activities. Further in vivo studies are needed to fully characterize its effects. |
| Enzyme Assay |
The in vitro enzyme assay for p-Ethynylphenylalanine hydrochloride involves measuring its inhibition of tryptophan hydroxylase activity. The assay typically uses tryptophan as a substrate and measures the production of 5-hydroxytryptophan. p-Ethynylphenylalanine hydrochloride is incubated with the enzyme and substrate at various concentrations. The Ki value is determined by fitting the inhibition data to a dose-response curve.
|
| Cell Assay |
In vitro cellular assays for p-Ethynylphenylalanine hydrochloride typically use cell lines that produce serotonin. Cells are treated with the compound at various concentrations. Serotonin levels in the cell lysate or medium are measured using HPLC or ELISA.
|
| Animal Protocol |
Animal/Disease Models: Male SD (Sprague-Dawley) rats (200 g)[1]
Doses: 30 mg/kg Route of Administration: intraperitoneal (ip) injection Experimental Results: diminished in 5-HT and 5-HIAA levels in the rat midbrain. In vivo animal experiments for p-Ethynylphenylalanine hydrochloride would typically use animal models to study serotonin function. The compound would be administered via intraperitoneal or oral routes. Serotonin levels in the brain and behavioral outcomes would be assessed. |
| ADME/Pharmacokinetics |
Pharmacokinetic properties of p-Ethynylphenylalanine hydrochloride are not extensively documented. As a small molecule with a molecular weight of 225.67, the compound is expected to have reasonable bioavailability. Its amino acid-like structure may influence its absorption, distribution, metabolism, and excretion.
|
| Toxicity/Toxicokinetics |
Toxicological data for p-Ethynylphenylalanine hydrochloride are not extensively available in the public domain. As a research compound, it has not undergone extensive toxicological evaluation. Standard cytotoxicity assays in cell lines may have been performed to assess safety margins.
|
| References | |
| Additional Infomation |
p-Ethynylphenylalanine hydrochloride (4-Ethynyl-L-phenylalanine hydrochloride) is a potent, selective, reversible, and competitive inhibitor of tryptophan hydroxylase with a Ki of 32.6 μM. It is used to study serotonin biosynthesis. The compound has a molecular weight of 225.67. No clinical trials or regulatory approvals have been reported.
|
| Molecular Formula |
C11H12CLNO2
|
|---|---|
| Molecular Weight |
225.671482086182
|
| Exact Mass |
225.055
|
| CAS # |
188640-63-3
|
| Related CAS # |
p-Ethynylphenylalanine;278605-15-5
|
| PubChem CID |
127264445
|
| Appearance |
Off-white to light yellow solid powder
|
| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
4
|
| Heavy Atom Count |
15
|
| Complexity |
245
|
| Defined Atom Stereocenter Count |
1
|
| SMILES |
Cl.OC([C@H](CC1C=CC(C#C)=CC=1)N)=O
|
| InChi Key |
MLHYNRVXXMXXQM-PPHPATTJSA-N
|
| InChi Code |
InChI=1S/C11H11NO2.ClH/c1-2-8-3-5-9(6-4-8)7-10(12)11(13)14;/h1,3-6,10H,7,12H2,(H,13,14);1H/t10-;/m0./s1
|
| Chemical Name |
(2S)-2-amino-3-(4-ethynylphenyl)propanoic acid;hydrochloride
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: 50 mg/mL (221.56 mM)
H2O: 12.5 mg/mL (55.39 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (11.08 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (11.08 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (11.08 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.4312 mL | 22.1562 mL | 44.3125 mL | |
| 5 mM | 0.8862 mL | 4.4312 mL | 8.8625 mL | |
| 10 mM | 0.4431 mL | 2.2156 mL | 4.4312 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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