| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| Other Sizes |
| Targets |
5-HT1A Receptor 5-HT1B Receptor 5-HT1D Receptor 5-HT5 Receptor 5-HT7 Receptor
5-Carboxamidotryptamine maleate targets multiple serotonin receptor subtypes. It is a potent agonist at 5-HT1A, 5-HT1B, 5-HT1D, 5-HT5A, and 5-HT7 receptors. By activating these receptors, it modulates serotonergic signaling pathways involved in various physiological processes. Its non-selective agonist profile makes it a valuable tool for studying the integrated effects of serotonin receptor activation. |
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| ln Vitro |
[3H] 5-Carboxamidotryptamine (5-CT) has low non-specific binding and high affinity (Kd = 0.38 nM) for 5-HT1D sites in the bovine substantia nigra. Its binding is also fast, reversible, and saturable[2]. [3H]-5-Carboxamidotryptamine (5-CT) tagged a similar number of binding sites (403 and 362 fmol/mg protein, respectively) in the bovine substantia nigra, and binding is guanine nucleotide-sensitive[2].
In vitro, 5-Carboxamidotryptamine maleate acts as a high-affinity agonist at multiple 5-HT receptor subtypes. Its activity is typically assessed by measuring its ability to stimulate cAMP accumulation or calcium mobilization in cells expressing various 5-HT receptors. The compound is a potent agonist with high affinity for its targets. Standard in vitro assays include receptor binding studies using radiolabeled ligands and functional assays measuring second messenger responses. |
| ln Vivo |
In anesthetized Wistar rats (serotonin depletion and treated with 20 mg/kg corticosterone), topical 5-carboxamidotryptamine (0.01-1000 μM) to the exposed dura mater encephala causes decreases in diastolic blood pressure, variable changes in meningeal blood flow, and increases in conductance (i.e. dilatation) in the middle meningeal artery[1].
In vivo, 5-Carboxamidotryptamine maleate is used in research to study serotonin receptor-mediated effects. Its non-selective agonist profile makes it useful for studying the integrated effects of serotonin receptor activation. However, its lack of selectivity limits its use in vivo for dissecting specific receptor functions. It is primarily used as a research tool in pharmacological studies. |
| Enzyme Assay |
For non-cell-based receptor binding assays, 5-Carboxamidotryptamine maleate can be evaluated using membrane preparations from tissues or cells expressing various 5-HT receptor subtypes. Radioligand binding displacement experiments are performed using suitable radiolabeled ligands such as [3H]-5-HT or [3H]-8-OH-DPAT. Membrane homogenates are incubated with increasing concentrations of the test compound and a fixed concentration of the radioligand. Bound radioligand is separated from free by rapid filtration. Non-specific binding is determined in the presence of excess unlabeled 5-HT. Ki values are calculated from displacement curves using nonlinear regression analysis.
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| Cell Assay |
For in vitro cellular assays, cells expressing various 5-HT receptor subtypes are cultured in appropriate media. For functional assays, the appropriate signaling readout is measured depending on the receptor subtype (e.g., cAMP accumulation for Gs-coupled receptors, calcium mobilization for Gq-coupled receptors). Cells are treated with various concentrations of 5-Carboxamidotryptamine maleate, and the signaling response is measured. EC50 values are calculated from dose-response curves.
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| Animal Protocol |
For in vivo animal studies, 5-Carboxamidotryptamine maleate can be administered to rodents via intraperitoneal injection. In models of serotonin-mediated behaviors, its effects on locomotion, thermoregulation, and pain perception are assessed. However, its lack of selectivity limits its use for dissecting specific receptor functions in vivo. Dosing regimens vary depending on the specific model.
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| ADME/Pharmacokinetics |
5-Carboxamidotryptamine maleate has a molecular weight of 319.31 and a molecular formula of C15H17N3O5. It is a synthetic tryptamine. The compound is supplied with a purity of ≥98% (HPLC). It should be stored at -20°C for long-term stability. It is soluble in DMSO and can be formulated for both in vitro and in vivo administration. It is for research use only and is not intended for human consumption.
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| Toxicity/Toxicokinetics |
The toxicity profile of 5-Carboxamidotryptamine maleate has not been extensively reported. As a non-selective serotonin receptor agonist, potential adverse effects may include modulation of serotonergic signaling. The compound is for research use only and is not intended for human consumption. Standard safety precautions should be observed when handling the compound.
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| References |
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| Additional Infomation |
5-Carboxamidotryptamine maleate (5-CT maleate, CAS 74885-72-6) is a non-selective, high-affinity agonist at serotonin (5-HT) receptors. It is a potent agonist at 5-HT1A, 5-HT1B, 5-HT1D, 5-HT5A, and 5-HT7 receptors. It is a synthetic compound that belongs to the class of tryptamines. It is available for research purposes only.
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| Molecular Formula |
C15H17N3O5
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|---|---|
| Molecular Weight |
319.312583684921
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| Exact Mass |
319.117
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| CAS # |
74885-72-6
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| Related CAS # |
5-Carboxamidotryptamine;74885-09-9
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| PubChem CID |
11709509
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| Appearance |
Solid powder
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| Hydrogen Bond Donor Count |
5
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
23
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| Complexity |
363
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C(/C(=O)O)=C/C(=O)O.C(C1=CNC2C=CC(C(=O)N)=CC1=2)CN
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 50 mg/mL (156.59 mM)
H2O: 25 mg/mL (78.29 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (7.83 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.1318 mL | 15.6588 mL | 31.3175 mL | |
| 5 mM | 0.6264 mL | 3.1318 mL | 6.2635 mL | |
| 10 mM | 0.3132 mL | 1.5659 mL | 3.1318 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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