| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| 5mg |
|
||
| 10mg | |||
| Other Sizes |
| Targets |
The primary molecular target of (RS)-AMPA monohydrate is the AMPA subtype of ionotropic glutamate receptors (AMPARs), which it activates as a potent agonist without interfering with binding sites for kainic acid or NMDA receptors.
|
|---|---|
| ln Vitro |
(RS)-AMPA monohydrate (10-3-10-4 M) causes the brainstem and spinal cord of cultivated rats to become depolarized. Although the effects of (RS)-AMPA monohydrate on depolarization differ greatly amongst neurons, it appears that the effects are dose-dependent. Applying (RS)-AMPA monohydrate at 10-5 M resulted in a very little depolarization (3–7 mV), however at 10–4 M, the depolarization's magnitude varied from 4–33 mV. The rate at which spontaneously firing neurons fire is also increased by (RS)-AMPA monohydrate, and quiet cells occasionally have short action potential bursts. Without impacting NMDA receptors, (RS)-AMPA monohydrate depolarizes the body by activating glutamate/quilamate receptors [1].
In cultured rat spinal and brainstem neurons, (RS)-AMPA monohydrate (10⁻⁵-10⁻⁴ M) induces dose-dependent depolarizations (3-7 mV at 10⁻⁵ M; 4-33 mV at 10⁻⁴ M), increases discharge rates of spontaneously firing neurons, and evokes short action potential bursts in silent cells by activating glutamate/quisqualate receptors without affecting NMDA receptors. |
| Enzyme Assay |
Binding assays for (RS)-AMPA monohydrate are typically performed using radiolabeled [³H]AMPA with rat brain membrane preparations or recombinant AMPA receptors; a standard saturation binding protocol involves incubating membranes with varying concentrations of [³H]AMPA (e.g., 5-100 nM) at 0-4°C for 60 minutes, with non-specific binding determined in the presence of excess unlabeled AMPA or L-glutamate.
|
| Cell Assay |
Cellular assays for (RS)-AMPA monohydrate are conducted using primary neuronal cultures; a representative protocol involves dissociating embryonic rat brainstem cells (E14), culturing them for 7-10 days, then treating with (RS)-AMPA monohydrate at concentrations ranging from 10⁻⁵ to 10⁻⁴ M for 3 days at different developmental stages, followed by cell survival assessment via counting gamma-enolase-positive neurons.
|
| References |
|
| Molecular Formula |
C7H12N2O5
|
|---|---|
| Molecular Weight |
204.180582046509
|
| Exact Mass |
204.074
|
| CAS # |
76463-67-7
|
| Related CAS # |
(S)-AMPA;83643-88-3;(RS)-AMPA;77521-29-0;(RS)-AMPA hydrobromide;171259-81-7
|
| PubChem CID |
53393722
|
| Appearance |
White to off-white solid powder
|
| Hydrogen Bond Donor Count |
4
|
| Hydrogen Bond Acceptor Count |
6
|
| Rotatable Bond Count |
3
|
| Heavy Atom Count |
14
|
| Complexity |
284
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
O1C(C)=C(C(N1)=O)CC(C(=O)O)N.O
|
| InChi Key |
HFISYNCCKQHIAM-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C7H10N2O4.H2O/c1-3-4(6(10)9-13-3)2-5(8)7(11)12;/h5H,2,8H2,1H3,(H,9,10)(H,11,12);1H2
|
| Chemical Name |
2-amino-3-(5-methyl-3-oxo-1,2-oxazol-4-yl)propanoic acid;hydrate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O: 5 mg/mL (24.49 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 7.14 mg/mL (34.97 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication (<60°C).
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.8976 mL | 24.4882 mL | 48.9764 mL | |
| 5 mM | 0.9795 mL | 4.8976 mL | 9.7953 mL | |
| 10 mM | 0.4898 mL | 2.4488 mL | 4.8976 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
