| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| 5mg |
|
||
| 10mg |
|
||
| Other Sizes |
|
| Targets |
Gli1-DNA Interaction[1]
|
|---|---|
| ln Vitro |
Glabrescione B (5 μM; 24-72 hours) suppresses the proliferation of Gli-dependent basal cell carcinoma[2]. Glabrescione B (1-10 μM; 24-48 hours) reduces Gli1 mRNA expression levels[2].
|
| Cell Assay |
Cell Proliferation Assay [1]
Cell Types: ASZ001 BCC cells Tested Concentrations: 5 μM Incubation Duration: 24-72 hrs (hours) Experimental Results: Basal cell carcinoma cell proliferation was impaired. Western Blot Analysis[1] Cell Types: ASZ001 BCC cells Tested Concentrations: 1-10 μM Incubation Duration: 24-48 hrs (hours) Experimental Results: Gli1 mRNA expression levels was decreased. |
| Animal Protocol |
Animal/Disease Models: Female NOD/SCID (severe combined immunodeficient) mouse (ASZ001 BCC allografts)[2]
Doses: 100 μmol/kg Route of Administration: Ip; every second day for 18 days Experimental Results: A significant reduction of tumor growth as well as Gli1 mRNA levels was observed. |
| References |
[1]. Ingallina C, et al. Polymeric glabrescione B nanocapsules for passive targeting of Hedgehog-dependent tumor therapy in vitro. Nanomedicine (Lond). 2017;12(7):711-728.
[2]. Infante P, et al. Gli1/DNA interaction is a druggable target for Hedgehog-dependent tumors. EMBO J. 2015;34(2):200-217. |
| Molecular Formula |
C27H30O6
|
|---|---|
| Molecular Weight |
450.52
|
| CAS # |
65893-94-9
|
| SMILES |
C/C(/C)=C\COC1=C(OC/C=C(\C)/C)C=CC(C2C(=O)C3C(=CC(OC)=CC=3OC)OC=2)=C1
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Solubility (In Vitro) |
DMSO: 100 mg/mL (221.97 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (5.55 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (5.55 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (5.55 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.2197 mL | 11.0983 mL | 22.1966 mL | |
| 5 mM | 0.4439 mL | 2.2197 mL | 4.4393 mL | |
| 10 mM | 0.2220 mL | 1.1098 mL | 2.2197 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
