| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
The primary targets of N-(p-Coumaroyl) Serotonin are not fully defined, but it is known to modulate inflammatory and oxidative stress pathways. It inhibits the production of proinflammatory cytokines by LPS-stimulated human blood monocytes. Its antioxidant activity suggests it may target reactive oxygen species (ROS) and other oxidative stress markers. It may also interact with serotonin receptors due to its serotonin moiety.
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| ln Vitro |
In vitro, N-(p-Coumaroyl) Serotonin has demonstrated antioxidant activity, protecting cells from oxidative stress. It has been shown to inhibit the production of proinflammatory cytokines in LPS-stimulated human blood monocytes. These activities are typically assessed using cell-based assays that measure cytokine levels, ROS production, and cell viability.
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| ln Vivo |
In vivo, N-(p-Coumaroyl) Serotonin has shown cardioprotective effects after ischemia. It may be beneficial in improving vascular function. Its antitumor activity has also been reported. These effects are attributed to its antioxidant and anti-inflammatory properties. Further studies are needed to fully elucidate its in vivo mechanisms.
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| Enzyme Assay |
Cell-free assays for N-(p-Coumaroyl) Serotonin typically involve measuring its antioxidant activity using chemical assays such as DPPH or ABTS radical scavenging assays. Its anti-inflammatory activity can be assessed using enzyme-linked immunosorbent assay (ELISA) to measure cytokine levels. These assays are used to characterize the compound's potency and mechanism of action.
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| Cell Assay |
In vitro cellular assays are conducted to evaluate the functional activity of N-(p-Coumaroyl) Serotonin. Immune cells, such as monocytes, are treated with the compound in the presence of LPS, and cytokine production is measured. Oxidative stress assays are used to measure the compound's ability to reduce ROS levels in cells. These assays confirm its anti-inflammatory and antioxidant activities.
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| Animal Protocol |
In vivo animal experiments typically involve models of ischemia-reperfusion injury to study its cardioprotective effects. Animals are administered the compound via oral gavage or injection. Markers of oxidative stress and inflammation are measured in tissues to assess efficacy. These studies provide evidence for the compound's therapeutic potential in cardiovascular diseases.
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| ADME/Pharmacokinetics |
The pharmacokinetic properties of N-(p-Coumaroyl) Serotonin are not well-characterized. As a naturally occurring compound, its absorption, distribution, metabolism, and excretion are likely influenced by its chemical structure. Its molecular weight is 322.36 g/mol. Further studies are needed to determine its bioavailability and half-life.
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| Toxicity/Toxicokinetics |
The toxicity profile of N-(p-Coumaroyl) Serotonin is not extensively documented. As a naturally occurring compound, it is generally considered to have low toxicity. However, standard safety precautions should be taken when handling it. Its use is restricted to research purposes and it is not intended for human consumption.
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| References |
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| Additional Infomation |
N6-cis-p-coumaroyl serotonin belongs to the hydroxyindole and carboxamide classes of compounds, and its function is related to serotonin. It has been reported that N-coumaroyl serotonin has been found in morning glory, safflower, and other organisms with relevant data.
N-(p-Coumaroyl) Serotonin (CS) is a naturally occurring compound with antioxidant, anti-inflammatory, cardioprotective, and antitumor activities. It is isolated from safflower seeds and millet grain. It is used in research to study oxidative stress, inflammation, and cardiovascular protection. It is not approved for therapeutic use and is intended for research purposes only. |
| Molecular Formula |
C19H18N2O3
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|---|---|
| Molecular Weight |
322.36
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| Exact Mass |
322.132
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| CAS # |
68573-24-0
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| PubChem CID |
5458879
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| Appearance |
Off-white to light yellow solid powder
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| Density |
1.346g/cm3
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| Boiling Point |
694.2ºC at 760 mmHg
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| Melting Point |
205 - 206 °C
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| Flash Point |
373.6ºC
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| Index of Refraction |
1.73
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| LogP |
3.342
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
24
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| Complexity |
446
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1=CC(=CC=C1/C=C/C(=O)NCCC2=CNC3=C2C=C(C=C3)O)O
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| InChi Key |
WLZPAFGVOWCVMG-FPYGCLRLSA-N
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| InChi Code |
InChI=1S/C19H18N2O3/c22-15-4-1-13(2-5-15)3-8-19(24)20-10-9-14-12-21-18-7-6-16(23)11-17(14)18/h1-8,11-12,21-23H,9-10H2,(H,20,24)/b8-3+
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| Chemical Name |
(E)-N-[2-(5-hydroxy-1H-indol-3-yl)ethyl]-3-(4-hydroxyphenyl)prop-2-enamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 250 mg/mL (775.53 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (6.45 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (6.45 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (6.45 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.1021 mL | 15.5106 mL | 31.0212 mL | |
| 5 mM | 0.6204 mL | 3.1021 mL | 6.2042 mL | |
| 10 mM | 0.3102 mL | 1.5511 mL | 3.1021 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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