| Size | Price | Stock | Qty |
|---|---|---|---|
| 1g |
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| Other Sizes |
| Targets |
HEPES does not have a specific biological target. It is a chemical buffer used to maintain pH. Its role is to provide a stable ionic environment for biological molecules and cells, not to modulate a biological pathway. It does not interact with biological targets in a specific, functional manner.
|
|---|---|
| ln Vitro |
For a minimum of three months, HEPES preserves titanium's superhydrophilicity, which leads to an ongoing retention of osteoconductivity and bioactivity[1]. In cultivated RAW264.7 cells, HEPES promotes lysosome formation, modifies the cytoplasmic-nuclear distribution of MiT/TFE, and modifies global cellular transcriptional patterns. These actions culminate in the activation of a MiT/TFE-dependent lysosomal-autophagic gene network[3].
In vitro, HEPES is used to buffer cell culture media (e.g., at a concentration of 10-25 mM) and various biochemical assay solutions. It does not have any intrinsic biological activity; its function is purely to stabilize pH. It is often preferred over bicarbonate buffers for experiments conducted in the absence of CO₂. |
| ln Vivo |
HEPES is not typically administered in vivo as a test compound. However, it is sometimes used as a buffer in formulations for intravenous or other parenteral administration of drugs in animal studies. In such cases, it serves as an excipient to maintain the pH of the formulation.
|
| Enzyme Assay |
HEPES is not used in enzyme/receptor binding assays as a test compound. It is a component of the assay buffer. Its role is to maintain a constant pH during the experiment, ensuring that the enzyme or receptor under study is in its optimal state.
|
| Cell Assay |
In cell culture, HEPES is added to the growth medium as a buffer. For example, many cell culture media are supplemented with 10-25 mM HEPES to provide additional buffering capacity, especially when cells are cultured in ambient air. It does not affect cell growth or viability at standard concentrations.
|
| Animal Protocol |
HEPES is not used as a test compound in animal studies. It may be included as an excipient in formulations of test compounds.
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| ADME/Pharmacokinetics |
HEPES is not a drug and is not subject to pharmacokinetic evaluation.
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| Toxicity/Toxicokinetics |
HEPES is generally recognized as safe (GRAS) for its intended use as a buffer in biological research and in some pharmaceutical formulations. It has low toxicity. Standard laboratory safety precautions should be followed.
|
| References | |
| Additional Infomation |
2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid is a HEPES (Hydroxylene-Electrolyte-1-ylene-2-ethylene-2-yl ...
HEPES is not a drug. It is a widely used biological buffer (Good's buffer) for cell culture and biochemical research. It is not approved for therapeutic use. |
| Molecular Formula |
C8H18N2O4S
|
|---|---|
| Molecular Weight |
238.30
|
| Exact Mass |
238.098
|
| CAS # |
7365-45-9
|
| Related CAS # |
HEPES sodium;75277-39-3;HEPES-d18;203805-87-2
|
| PubChem CID |
23831
|
| Appearance |
White to off-white solid powder
|
| Density |
1.3±0.1 g/cm3
|
| Melting Point |
234-238 °C
|
| Index of Refraction |
1.543
|
| LogP |
-2.67
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
6
|
| Rotatable Bond Count |
5
|
| Heavy Atom Count |
15
|
| Complexity |
267
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
C1CN(CCN1CCO)CCS(=O)(=O)O
|
| InChi Key |
JKMHFZQWWAIEOD-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
|
| Chemical Name |
2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O: 250 mg/mL (1049.10 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (419.64 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.1964 mL | 20.9820 mL | 41.9639 mL | |
| 5 mM | 0.8393 mL | 4.1964 mL | 8.3928 mL | |
| 10 mM | 0.4196 mL | 2.0982 mL | 4.1964 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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