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| Other Sizes |
| Targets |
HEPES sodium does not have a specific biological target as a drug. It is a zwitterionic buffer used to maintain pH in biological experiments. Good's buffers are characterized by high water solubility, low cell membrane permeability, consistent acid-base dissociation constants, low metal chelating capability, and high chemical stability. HEPES is used in cell culture, protein chemistry, and various biochemical assays to maintain a stable pH environment.
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| ln Vitro |
For a minimum of three months, HEPES can preserve titanium's superhydrophilicity while preserving its bioactivity and osteoconductivity [1]. In cultivated RAW264.7 cells, HEPES promotes lysosomal biosynthesis, modifies the cytoplasmic-nuclear distribution of MiT/TFE, upends the global cellular transcriptional profile, and therefore activates the gene network for MiT/TFE-dependent lysosomal autophagy [3].
HEPES sodium does not have direct in vitro biological activity as a pharmacological agent. Its value is as a buffer for biochemical and cell biology research. The compound maintains a stable pH environment for experiments without interfering with the biochemical processes being studied. It is used in buffer solutions for various in vitro applications including cell culture, enzyme assays, and protein purification. |
| ln Vivo |
HEPES sodium does not have in vivo biological activity as a pharmacological agent. It is not intended for administration to animals or humans as a therapeutic. Its value is as a laboratory reagent for biochemical research. The compound is used in buffer solutions for various in vitro and ex vivo applications. It is not administered in vivo.
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| Enzyme Assay |
In vitro biochemical assays using HEPES buffer are performed to maintain pH during experiments. The buffer is prepared at the desired pH within its effective range (6.8-8.2). HEPES is used in cell culture media, protein purification, enzyme assays, and electrophoresis. It is also used in tissue or organ culture to maintain pH.
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| Cell Assay |
Cell culture experiments using HEPES sodium are common, as it is a preferred buffer for maintaining physiological pH in cell culture media. Cells are cultured in media containing HEPES buffer at the appropriate concentration (typically 10-25 mM). The buffer maintains pH during experiments outside of CO₂ incubators. HEPES is also used in cell lysis buffers and other applications.
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| Animal Protocol |
In vivo animal experiments are not applicable to HEPES sodium as it is a buffer reagent rather than a therapeutic agent. The compound is not intended for administration to animals. Its value is as a laboratory reagent for biochemical and cell biology research. Researchers should not attempt in vivo administration of this compound.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties are not applicable to HEPES sodium as it is a buffer reagent, not a therapeutic agent. The compound is not intended for administration to animals or humans. Its physicochemical properties include a molecular weight of 260.29, a molecular formula of C8H17N2NaO4S, and a buffering range of pH 6.8-8.2. It is highly water-soluble. Storage: room temperature, protected from moisture.
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| Toxicity/Toxicokinetics |
HEPES sodium is considered a safe buffer for laboratory use with minimal toxicity at typical working concentrations. Good's buffers are designed for minimal interference with biochemical processes. However, as with all chemical reagents, appropriate safety precautions should be followed. The compound is for research use only and is not for human therapeutic use. No specific toxicity data are available.
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| References | |
| Additional Infomation |
Sodium 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate is an organosodium salt composed of N-(2-hydroxyethyl)piperazine and N-(sulfoalkyl)piperazine. It contains 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate.
HEPES sodium has CAS number 75277-39-3, molecular formula C8H17N2NaO4S, and molecular weight 260.29. It is a zwitterionic biological buffer used to maintain pH in biological and biochemical research. It has a buffering range of pH 6.8-8.2. Purity: ≥99%. Not for human use; for research purposes only. |
| Molecular Formula |
C8H17N2NAO4S
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|---|---|
| Molecular Weight |
260.2864
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| Exact Mass |
260.08
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| CAS # |
75277-39-3
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| Related CAS # |
HEPES;7365-45-9
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| PubChem CID |
2724248
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| Appearance |
White to off-white solid powder
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| Melting Point |
234ºC
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
16
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| Complexity |
272
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1CN(CCN1CCO)CCS(=O)(=O)[O-].[Na+]
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| InChi Key |
RDZTWEVXRGYCFV-UHFFFAOYSA-M
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| InChi Code |
InChI=1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1
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| Chemical Name |
sodium;2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~25 mg/mL (~96.05 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (9.60 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (9.60 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (9.60 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.8419 mL | 19.2093 mL | 38.4187 mL | |
| 5 mM | 0.7684 mL | 3.8419 mL | 7.6837 mL | |
| 10 mM | 0.3842 mL | 1.9209 mL | 3.8419 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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