| Size | Price | Stock | Qty |
|---|---|---|---|
| 25g |
|
||
| Other Sizes |
| Targets |
Phenol Red does not target a specific protein or enzyme. Its function as a pH indicator is based on its chemical properties as a weak acid that undergoes a color change upon protonation or deprotonation in response to changes in hydrogen ion concentration.
|
|---|---|
| ln Vitro |
For a fast assessment of the culture's health, phenol red (PR), the standard pH indicator in a variety of cell and tissue culture media, is used. The detection of cellular hydrogen peroxide and human peroxidase enzyme activity has also been accomplished through the use of phenol red in a number of protocols[1].
The in vitro activity of Phenol Red is its ability to function as a pH indicator. In cell culture media, it provides a visual indication of pH changes: at pH 6.4 or below it appears yellow, and at pH 8.2 or above it appears red. This property makes it valuable for monitoring the health and metabolic status of cell cultures. |
| ln Vivo |
Phenol Red is not used as a therapeutic agent and has no significant in vivo pharmacological activity. Its primary application is as an indicator in diagnostic and research settings. The compound is incorporated into various culture media to monitor pH changes.
|
| Enzyme Assay |
In vitro binding assays are not applicable for Phenol Red as it functions as a pH indicator rather than a drug targeting specific enzymes or receptors. The compound is used in colorimetric assays where its color change in response to pH is measured spectrophotometrically.
|
| Cell Assay |
For in vitro cellular experiments, Phenol Red is incorporated into cell culture media at appropriate concentrations. It allows researchers to visually monitor pH changes in the culture environment, providing an indication of cell metabolism and medium condition. The dye can be detected spectrophotometrically for quantitative pH measurements.
|
| Animal Protocol |
In vivo animal experiments with Phenol Red are not typically performed as a therapeutic intervention. The compound may be used in physiological studies to track pH changes in biological fluids or as a tracer in certain experimental procedures.
|
| ADME/Pharmacokinetics |
Pharmacokinetic properties of Phenol Red have not been characterized, as the compound is a research-use indicator rather than a drug candidate. It is soluble in water and should be stored under appropriate conditions. The compound has been used as a diagnostic indicator in various applications.
|
| Toxicity/Toxicokinetics |
Toxicological data for Phenol Red indicates that it is generally regarded as safe for use as a pH indicator in cell culture and diagnostic applications. However, the compound is not intended for human therapeutic use. Standard laboratory safety precautions should be observed during handling.
|
| References | |
| Additional Infomation |
Phenol red is 3H-2,1-benzoxoxetane-1,1-dioxide, in which both hydrogen atoms at the 3-position are replaced by 4-hydroxyphenyl groups. It is a pH indicator, appearing yellow below pH 6.8 and bright pink above pH 8.2. Phenol red is commonly used in cell culture and home swimming pool water quality testing kits. It was also used (now rarely) in the phenolsulfonphthalein (PSP) test to assess total renal blood flow. Phenol red has dual uses as a bicolor indicator, acid-base indicator, and diagnostic reagent. It is a 2,1-benzoxoxetane, belonging to the phenol, sulfonyl lactone, and aromatic sulfonate classes. Phenolsulfonphthalein, also known as phenol red, is a commonly used pH indicator widely used in cell biology laboratories. Due to its weak estrogen-mimicking properties, phenolsulfonphthalein is being investigated for clinical applications. It is a red dye, pH indicator, and adjunct to renal function diagnostics. It is also used in research on the gastrointestinal tract and other systems.
Phenol Red is a sulfonphthalein pH indicator widely used in biology and medicine. It is commonly used to detect pH changes in cell culture and is incorporated into microbiological culture media for the identification of Escherichia coli strains. The compound has no clinical or therapeutic applications and has not received regulatory approval for systemic use. |
| Molecular Formula |
C19H14O5S
|
|---|---|
| Molecular Weight |
354.38
|
| Exact Mass |
354.056
|
| CAS # |
143-74-8
|
| Related CAS # |
Phenol Red sodium salt;34487-61-1
|
| PubChem CID |
4766
|
| Appearance |
Red to reddish brown solid powder
|
| Density |
1.5±0.1 g/cm3
|
| Boiling Point |
562.8±50.0 °C at 760 mmHg
|
| Melting Point |
300 °C
|
| Flash Point |
294.2±30.1 °C
|
| Vapour Pressure |
0.0±1.6 mmHg at 25°C
|
| Index of Refraction |
1.697
|
| LogP |
3.02
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
5
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
25
|
| Complexity |
545
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
C1=CC=C2C(=C1)C(OS2(=O)=O)(C3=CC=C(C=C3)O)C4=CC=C(C=C4)O
|
| InChi Key |
BELBBZDIHDAJOR-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C19H14O5S/c20-15-9-5-13(6-10-15)19(14-7-11-16(21)12-8-14)17-3-1-2-4-18(17)25(22,23)24-19/h1-12,20-21H
|
| Chemical Name |
4-[3-(4-hydroxyphenyl)-1,1-dioxo-2,1λ6-benzoxathiol-3-yl]phenol
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: 50 mg/mL (141.09 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (7.05 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (7.05 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.8218 mL | 14.1091 mL | 28.2183 mL | |
| 5 mM | 0.5644 mL | 2.8218 mL | 5.6437 mL | |
| 10 mM | 0.2822 mL | 1.4109 mL | 2.8218 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
