Size | Price | Stock | Qty |
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1mg |
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Other Sizes |
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Targets |
Amyloid-β[1]
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ln Vitro |
β-Amyloid Aggregation Guidelines: The methodology that follows is advised; it should be adjusted to meet your unique requirements as it merely serves as a guide. 1. In cold hexafluoro-2-propanol (HFIP) medium, dissolve solid Aβ peptide. To achieve monomerization and randomization of the structure, incubate the peptides for a minimum of one hour at room temperature. 2. The resultant peptide should be stored in thin film form at -20 or -80°C after being evaporated to remove HFIP. 3. After the membrane has been dissolved, vortex and dilute it to the proper concentration and buffer (serum- and phenol red-free media) using 5 mM anhydrous DMSO. 4. After that, age the mixture for 48 hours at 4–8°C. After centrifuging the samples for 10 minutes at 4–8°C at 14,000 g, the supernatant contained soluble oligomers. For the studies, the supernatant was diluted 10-200 times. Different approaches are used based on the final application.
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References |
[1]. Mozes E, et al. A novel method for the rapid determination of beta-amyloid toxicity on acute hippocampal slices using MTT and LDH assays. Brain Res Bull. 2012 Apr 10;87(6):521-5.
[2]. Lagunes T, et al. Abeta(1-42) induces abnormal alternative splicing of tau exons 2/3 in NGF-induced PC12 cells. An Acad Bras Cienc. 2014 Dec;86(4):1927-34. [3]. Stefania Sabella, et al. Capillary electrophoresis studies on the aggregation process of beta-amyloid 1-42 and 1-40 peptides. Electrophoresis. 2004 Oct;25(18-19):3186-94. |
Molecular Formula |
C199H307N53O59S.C2HF3O2
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Molecular Weight |
4532.04
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Related CAS # |
β-Amyloid (1-42), (rat/mouse);166090-74-0
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product is not stable in solution, please use freshly prepared working solution for optimal results. (2). Please store this product in a sealed and protected environment, avoid exposure to moisture. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO :~55 mg/mL (~12.14 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (0.55 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 0.2207 mL | 1.1033 mL | 2.2065 mL | |
5 mM | 0.0441 mL | 0.2207 mL | 0.4413 mL | |
10 mM | 0.0221 mL | 0.1103 mL | 0.2207 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.