| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
Ca2+ channels. It also targets transcription factors involved in adipogenesis.
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| ln Vitro |
3,5,7,3′,4′-Pentamethoxyflavone is a Ca2+ channel inhibitor. It induces relaxation of the human corpus cavernosum through calcium mobilization-related mechanisms. In 3T3-L1 preadipocytes, it stimulates adipogenesis by controlling transcription factors during early differentiation. It can protect DNA from oxidative damage.
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| ln Vivo |
In vivo, while specific studies for this compound are limited, related polymethoxyflavones are known to have various biological effects, including anti-inflammatory and metabolic activities. Its ability to induce adipogenesis suggests potential applications in metabolic research.
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| Enzyme Assay |
The inhibition of Ca2+ channels can be assessed using patch-clamp electrophysiology on cells expressing the channels. The compound's effect on calcium mobilization can also be measured using fluorescent calcium indicators (e.g., Fura-2) in cell-based assays. Its ability to protect DNA from oxidative damage can be assessed in vitro using a DNA cleavage assay with a reactive oxygen species (ROS) generator.
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| Cell Assay |
To study its effect on adipogenesis, 3T3-L1 preadipocytes are cultured and treated with the compound during the early stages of differentiation. After the differentiation period, adipogenesis is assessed by Oil Red O staining, which quantifies lipid accumulation. The expression of adipogenic transcription factors (e.g., PPARγ, C/EBPα) is measured by qPCR or Western blot.
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| Animal Protocol |
Specific in vivo data for 3,5,7,3′,4′-Pentamethoxyflavone are limited. However, its effects on adipogenesis could be studied in mouse models of obesity or metabolic syndrome. The compound could be administered orally or intraperitoneally to assess its effects on body weight, adipose tissue mass, and metabolic parameters.
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| ADME/Pharmacokinetics |
Specific PK data for this compound are not widely published. As a small molecule (MW 372.12) and a flavonoid, it is expected to be absorbed after oral administration. Its metabolism and bioavailability would be typical of polyphenolic compounds, often undergoing extensive phase II metabolism.
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| Toxicity/Toxicokinetics |
Specific toxicological data are not available. As a natural product, it is generally considered to have low toxicity. However, comprehensive safety studies are lacking. Standard laboratory safety precautions should be followed when handling the pure compound.
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| References | |
| Additional Infomation |
According to reports, quercetin pentamethyl ether is found in Kaempferia trifoliata, Kaempferia galanga, and other organisms with available data.
3,5,7,3′,4′-Pentamethoxyflavone is a research compound used to study various biological processes. Its role as a Ca2+ channel inhibitor and its ability to modulate adipogenesis make it a tool for investigating cardiovascular function and metabolic diseases. It is a natural product, and its study contributes to the understanding of the pharmacological activities of plant-derived compounds. |
| Molecular Formula |
C20H20O7
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|---|---|
| Molecular Weight |
372.37
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| Exact Mass |
372.121
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| CAS # |
1247-97-8
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| PubChem CID |
97332
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| Appearance |
White to light brown solid
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| Density |
1.29 g/cm3
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| Boiling Point |
561.9ºC at 760 mmHg
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| Melting Point |
152-156ºC
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| Flash Point |
246.9ºC
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| Vapour Pressure |
1.18E-12mmHg at 25°C
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| Index of Refraction |
1.588
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| LogP |
3.503
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
27
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| Complexity |
561
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O1C(C2C([H])=C([H])C(=C(C=2[H])OC([H])([H])[H])OC([H])([H])[H])=C(C(C2C(=C([H])C(=C([H])C1=2)OC([H])([H])[H])OC([H])([H])[H])=O)OC([H])([H])[H]
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| InChi Key |
ALGDHWVALRSLBT-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C20H20O7/c1-22-12-9-15(25-4)17-16(10-12)27-19(20(26-5)18(17)21)11-6-7-13(23-2)14(8-11)24-3/h6-10H,1-5H3
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| Chemical Name |
2-(3,4-dimethoxyphenyl)-3,5,7-trimethoxychromen-4-one
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| Synonyms |
3,5,7,3′,4′-Pentamethoxyflavone
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 25 mg/mL (67.14 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.71 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (6.71 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6855 mL | 13.4275 mL | 26.8550 mL | |
| 5 mM | 0.5371 mL | 2.6855 mL | 5.3710 mL | |
| 10 mM | 0.2686 mL | 1.3428 mL | 2.6855 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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