| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| 10mg |
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| 50mg |
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| Other Sizes |
| ln Vitro |
SK2188 (10-10000 nM, 48-120 h) effectively inhibited the proliferation of MYCN-amplified cells and was effective against organoid cells derived from neuroblastoma (NB) patients [1]. SK2188 (10-1000 nM, 24 h) inhibited cell growth by inducing apoptosis in NGP cells [1]. SK2188 (10-1000 nM, 24 h) degraded AURKA in NGP cells, thereby inducing DNA damage and replication stress [1]. SK2188 (100-1000 nM, 3 h) showed high targeting selectivity and degradation specificity in NGP cells [1].
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| Cell Assay |
Cell viability assay [1]
Cell Types: NGP cells, IMR-32 cells, N206 cells, SK-N-AS cells, organoids derived from NB patients Tested Concentrations: 10 nM, 100 nM, 1000 nM, 10000 nM Incubation Duration: 48 hours (NGP cells, IMR-32 cells, N206 cells, SK-N-AS cells); 120 hours (organoids derived from NB patients) Experimental Results: In NGP and IMR-32 cells, the IC50 values for inhibiting cell viability were 31.9 nM and 21.5 nM, respectively, which were significantly lower than the IC50 values of their parent inhibitor MK-5108 (348.9 nM and 199.3 nM, respectively). In the MYCN-amplified organoids NB139 and NB067, the IC50 values for inhibiting cell viability were 131.3 nM and 26.4 nM, respectively, which were significantly lower than the IC50 values of their parent inhibitor MK-5108 (899.7 nM and 75.3 nM, respectively). Apoptosis analysis [1] Cell Types: NGP cells Tested Concentrations: 10 nM, 100 nM, 1000 nM Incubation Duration: 24 hours Experimental Results: At 10 nM, it significantly activated Caspase 3/7 and at 1000 nM, it had a stronger apoptosis-inducing effect than MK-5108. Western Blot Analysis [1] Cell Types: NGP Neuroblastoma Cells Tested Concentrations: 10 nM, 100 nM, 500 nM, 1000 nM Incubation Duration: 1 hour, 3 hours, 6 hours, 24 hours, 48 hours, 72 hours Experimental Results: After 24 hours, the degradation rate of AURKA reached 89% (DC50 = 3.9 nM). The level of MYCN protein decreased (73% degradation after 72 hours). After 24 hours, the expression of DNA damage markers (γH2AX, pCHK1) increased. |
| References |
| Molecular Formula |
C43H46CLFN6O10S
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|---|---|
| Molecular Weight |
893.38
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| CAS # |
3038446-94-2
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| Appearance |
White to off-white solid
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| SMILES |
O=C(N1C2C(NC(CC2)=O)=O)C3=CC=CC(OCCOCCOCCOCCNC([C@@]4(CC[C@H](CC4)OC5=C(C(Cl)=CC=C5)F)CC6=CC=CC(NC7=NC=CS7)=N6)=O)=C3C1=O
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| Synonyms |
JB325
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~111.93 mM; with sonication)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (2.80 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you canAdd 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of physiological saline: Dissolve 0.9 g of sodium chloride in double-distilled water and dilute to 100 mL to obtain clear physiological saline. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (2.80 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you canAdd 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.1193 mL | 5.5967 mL | 11.1934 mL | |
| 5 mM | 0.2239 mL | 1.1193 mL | 2.2387 mL | |
| 10 mM | 0.1119 mL | 0.5597 mL | 1.1193 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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