| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| Other Sizes |
| ln Vitro |
PhpC enhances the reverse transcription of rMYC G4 RNA while significantly inhibiting the reverse transcription of rhTELO, r5YEY, and rTBA[3]. PhpC promotes the in vitro translation of G4 RNA[3]. PhpC inhibits the viability of HEK293FT cells with an IC50 value of 25.7 μM[3]. PhpC (5-20 μM; 24 h) induces an increase in the DNA damage marker γH2AX, indicating its ability to induce DNA damage[3]. PhpC (25-100 μM; 24 h) leads to a gradual increase in the transcription level of mature miR-3196 in A549 cells[4]. PhpC recognizes and binds to the region constituting the G4s (rG4)-3196 sequence, disrupting its native G4 conformation and thus causing a structural change[4]. In HeLa cells, immunofluorescence (IF) analysis showed that 100 µM PhpC significantly reduced G4 levels but had no effect on metabolic activity [5]. PhpC (90 µM, 48 h) significantly reduced the abundance of NRAS G4 in MCF7 cells [6]. PhpC (90 μM; 72 h) had no significant effect on LINC01589 and MELTF-AS1, but significantly reduced the expression of UXT-AS1 and NRAS in HT-29 cells [7]. PhpC regulates G4 distribution in a targeted manner, alters translational activity in astrocytes only, and upregulates 295 proteins that are major participants in RNA transcription [8].
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|---|---|
| Cell Assay |
RT-PCR[4]
Cell Types: A549 cells Tested Concentrations: 25 μM, 50 μM, 100 μM Incubation Duration: 24 hours Experimental Results: Effectively increased the level of miR-3196 in cells. |
| References |
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| Molecular Formula |
C18H20N4O4
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|---|---|
| Molecular Weight |
356.38
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| Appearance |
Light yellow to yellow solid
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| SMILES |
NCCOC1=CC=CC(C2=CC3=CN(C(N=C3N2)=O)CC(OCC)=O)=C1
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: 请将本产品存放在密封且受保护的环境中（例如氮气下），避免暴露在潮湿和光照下。 |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~280.60 mM; with sonication)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (7.01 mM) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Suspended solution; Need ultrasonic.
For example, if 1 mL of working solution is to be prepared, you canAdd 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of physiological saline: Dissolve 0.9 g of sodium chloride in double-distilled water and dilute to 100 mL to obtain clear physiological saline. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (7.01 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Suspended solution; Need ultrasonic. For example, if 1 mL of working solution is to be prepared, you canAdd 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD saline (4°C, store for one week): Dissolve 2 g of SBE-β-CD powder in 10 mL of saline until completely dissolved and clear. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: 2.5 mg/mL (7.01 mM) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Suspended solution; Need ultrasonic. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.8060 mL | 14.0300 mL | 28.0599 mL | |
| 5 mM | 0.5612 mL | 2.8060 mL | 5.6120 mL | |
| 10 mM | 0.2806 mL | 1.4030 mL | 2.8060 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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