| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
| ln Vitro |
NC03 (15-76 μM; 1 h) effectively inhibited the activity of human PI4K2A enzyme expressed by bacteria in COS-7 cells [1]. NC03 (10 μM; pre-incubation 10 min, kinase reaction 1.5 h) at a concentration of 10 μM inhibited the activity of palmitoylated PI4K2A enzyme isolated from COS-7 cells [1]. NC03 (1-10 μM; pre-incubation 20 min, permeabilized cell incubation 30 min) inhibited PI4K2A-mediated PI4P synthesis in permeabilized COS-7 cells and had a secondary effect on PI(4,5)P2 levels [1]. NC03 (10 μM; up to 10 min) at a concentration of 10 μM rapidly reduced the PI4P content in the Golgi apparatus and endosomes of live COS-7 cells [1]. NC03 (10–30 μM; 30 min) reduced the level of Rab7-positive late endosome PI4P in intact HEK-AT1 cells in a concentration-dependent manner, with 30 μM being more potent than 10 μM [1].
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|---|---|
| Cell Assay |
Cell viability assay [1]
Cell Types: COS-7 Tested Concentrations: 15, 76 μM Incubation Duration: 1 hour Experimental Results: The activity of human PI4K2A enzyme expressed by bacteria was inhibited, and its efficacy was related to the activity of palmitoylated PI4K2A in COS-7 cells. |
| References |
|
| Molecular Formula |
C21H21N3O7S
|
|---|---|
| Molecular Weight |
459.47
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| CAS # |
568558-66-7
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| Appearance |
White to off-white solid
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| SMILES |
O=C(CSC1=NN=C(C2=CC(OC)=C(OC)C(OC)=C2)O1)NC3=CC=C4OCCOC4=C3
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~217.64 mM; with sonication)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.44 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you canAdd 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1764 mL | 10.8821 mL | 21.7642 mL | |
| 5 mM | 0.4353 mL | 2.1764 mL | 4.3528 mL | |
| 10 mM | 0.2176 mL | 1.0882 mL | 2.1764 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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