| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| Other Sizes |
| ln Vitro |
Human acetylgastrin (TFA) induces the production of cyclic adenosine monophosphate (cAMP) and has an EC50 value of 1.9 nM in Chinese hamster lung fibroblasts transfected with human GIP receptor[1]. Human acetylgastrin (TFA) (10-13-10-8 nM) showed a stronger stimulatory effect on insulin release in BRIN-BD11 cells compared with native GIP[1]. Human acetylgastrin (TFA) can improve glucose intolerance, type 2 diabetes, decreased β-cell glucose sensitivity, insulin resistance and decreased insulin secretion[2]. Human acetylgastrin (TFA) is metabolically stable and its hypoglycemic and insulin-regulating activities have been evaluated in vitro and in vivo for two fatty acid-derived N-terminal acetylated GIP analogs[3].
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| ln Vivo |
Acetyl gastric inhibitory peptide (TFA) (25 nmol/kg; intraperitoneal injection; single dose) is resistant to plasma dipeptidyl peptidase IV degradation, thereby enhancing its biological activity and improving its anti-diabetic potential in vivo [1].
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| References |
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| Molecular Formula |
C228H340N60O67S.C2HF3O2
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|---|---|
| Molecular Weight |
5139.62
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| Related CAS # |
Acetyl Gastric Inhibitory Peptide (human)
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| Sequence |
Ac-Tyr-Ala-Glu-Gly-Thr-Phe-Ile-Ser-Asp-Tyr-Ser-Ile-Ala-Met-Asp-Lys-Ile-His-Gln-Gln-Asp-Phe-Val-Asn-Trp-Leu-Leu-Ala-Gln-Lys-Gly-Lys-Lys-Asn-Asp-Trp-Lys-His-Asn-Ile-Thr-GlnAc-YAEGTFISDYSIAMDKIHQQDFVNWLLAQKGKKNDWKHNITQ
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| SequenceShortening |
Ac-YAEGTFISDYSIAMDKIHQQDFVNWLLAQKGKKNDWKHNITQ
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| Appearance |
White to off-white solid
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| Synonyms |
Human N-acetyl GIP TFA
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: 请将本产品存放在密封保护的环境中，避免受潮。 |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~19.46 mM; with sonication)
H2O : ~50 mg/mL (~9.73 mM; with sonication) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (0.49 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you canAdd 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of physiological saline: Dissolve 0.9 g of sodium chloride in double-distilled water and dilute to 100 mL to obtain clear physiological saline. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (0.49 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you canAdd 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.1946 mL | 0.9728 mL | 1.9457 mL | |
| 5 mM | 0.0389 mL | 0.1946 mL | 0.3891 mL | |
| 10 mM | 0.0195 mL | 0.0973 mL | 0.1946 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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