| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
| ln Vitro |
dAurAB5 (500 nM, 24 hours) can induce significant degradation of Aurora-A and Aurora-B, with degradation rates of 84% and 82%, respectively[1]. dAurAB5 (500 nM, 24 hours) can reduce the level of N-Myc in IMR32 cells by 45%[1]. dAurAB5 (100 nM, 4 hours) can degrade Aurora-A by 83% and Aurora-B by 95% in IMR32 cells[1]. dAurAB5 (200 nM, 6 hours) can reduce the abundance of Aurora-A and the levels of AAK1, PTK2, GAK, and TTK in Kelly neuroblastoma cells amplified by MYCN, but does not downregulate the abundance of Aurora-B[1]. dAurAB5 (0-1000 nM, 24 hours) can reduce the cell viability of IMR32 cells, but has very little cytotoxicity to HEK293 cells[1].
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|---|---|
| Cell Assay |
Western Blot Analysis[1]
Cell Types: IMR32 cells Tested Concentrations: 500 nM Incubation Duration: 24 h Experimental Results: Reduced N-Myc levels by 45%. Western Blot Analysis[1] Cell Types: IMR32 cells Tested Concentrations: 100 nM Incubation Duration: 4 h Experimental Results: Degraded 83% of Aurora-A and 95% of Aurora-B. Cell Viability Assay[1] Cell Types: IMR32 cells Tested Concentrations: 0, 10, 100, 1000 nM Incubation Duration: 24 h Experimental Results: Significant reduced cell viability, with 45% reduction in cell viability at 100 nM and 55% reduction in cell viability at 1 μM. |
| References |
| Molecular Formula |
C42H49N11O7
|
|---|---|
| Molecular Weight |
819.91
|
| Appearance |
Yellow to orange solid powder
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~121.96 mM; with sonication)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 5 mg/mL (6.10 mM)(saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one)),clear solution.
For example, if 1 mL of working solution is to be prepared, you can Add 100 μL of 50.0 mg/mL clarified DMSO stock solution to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.2196 mL | 6.0982 mL | 12.1965 mL | |
| 5 mM | 0.2439 mL | 1.2196 mL | 2.4393 mL | |
| 10 mM | 0.1220 mL | 0.6098 mL | 1.2196 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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