| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
|
||
| 10mg |
|
||
| 50mg |
|
||
| Other Sizes |
| ln Vitro |
D574-0246 (4 days) reduced the survival rate of PLC cells with an IC50 of 16.49 μM and reduced the survival rate of HepG2 cells with an IC50 of 6.656 μM[1]. D574-0246 (1-10 μM) significantly reduced substrate-specific (LACTB K284) and global protein succinylation in HepG2 cells in a dose-dependent manner[1]. D574-0246 (1-10 μM) significantly reduced the ketogenic activity of OXCT1 in HepG2 cells[1].
|
|---|---|
| ln Vivo |
D574-0246 (55 mg/kg, intraperitoneal injection, once every 3 days for a total of 8 times) significantly inhibited tumor growth in nude mice with xenografts of HepG2 overexpressing OXCT1 [1].
|
| Animal Protocol |
Animal/Disease Models: HepG2 cells with OXCT1 overexpression or knockout (2 × 106) were subcutaneously implanted into the flanks of 5-week-old male BALB/c nude mice[1]
Doses: 55 mg/kg Route of Administration: i.p., once every 3 days for 8 times Experimental Results: Inhibited tumor growth in nude mice bearing OXCT1-overexpressing HepG2 xenograft tumors, while exerting no significant inhibitory effect on tumor growth in nude mice with OXCT1-knockout HepG2 xenograft tumors. Reduced both global protein succinylation levels and LACTB K284-specific succinylation levels in OXCT1-overexpressing tumor tissues. Showed no significant changes in body weight. Caused no significant abnormalities in serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) in nude mice. Induced no obvious morphological changes in major organs (liver, kidney, and spleen) of nude mice. |
| References |
| Molecular Formula |
C24H26N6O3
|
|---|---|
| Molecular Weight |
446.50
|
| CAS # |
1269110-12-4
|
| Appearance |
White to light yellow solid powder
|
| Synonyms |
iOXCT1
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~223.96 mM; with sonication)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 5 mg/mL (11.20 mM)(saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one)),clear solution.
For example, if 1 mL of working solution is to be prepared, you can Add 100 μL of 50.0 mg/mL clear DMSO stock solution to 400 μL of PEG300 and mix thoroughly. Then add 50 μL of Tween-80 to the above system and mix thoroughly. Finally, add 450 μL of physiological saline to bring the volume to 1 mL. Preparation of physiological saline: Dissolve 0.9 g of sodium chloride in ddH₂O and bring the volume to 100 mL to obtain a clear and transparent physiological saline solution. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 5 mg/mL (11.20 mM)(saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one)),clear solution. For example, if 1 mL of working solution is to be prepared, you can Add 100 μL of 50.0 mg/mL clarified DMSO stock solution to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.2396 mL | 11.1982 mL | 22.3964 mL | |
| 5 mM | 0.4479 mL | 2.2396 mL | 4.4793 mL | |
| 10 mM | 0.2240 mL | 1.1198 mL | 2.2396 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
