| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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Praeruptorin B, a naturally occuring compound from roots of Peucedanum praeruptorum Dunn, is a potent inhibitor of sterol regulatory element-binding proteins (SREBPs).
| ln Vitro |
Praeruptorin B suppresses SREBP activity and decreases intracellular lipid levels. Studies have demonstrated that Praeruptorin B can dramatically suppress SRE luciferase activity, and this impact is dose-dependent. Even at greater concentrations, the cytotoxicity of Praeruptorin B was low. Praeruptorin B also drastically downregulates the expression of SREBP-1c and SREBP-2 [1]. Praeruptorin B also demonstrates considerable inhibitory effects on the activity of UGT1A9 [2].
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| ln Vivo |
Although they were remained heavier than diet-fed mice, mice treated with Praeruptorin B (50 mg/kg) were noticeably lighter than mice treated with a vehicle, indicating that Praeruptorin B may be able to lessen diet-induced obesity (DIO). More notably, mice treated with the same amount of Praeruptorin B showed a significant reduction in both the fat/lean and fat/body weight ratios. Additionally, it was demonstrated that animals treated with prateruptorin B had much lower serum levels of TC and TG than mice fed a high-fat diet. Similar to lovastatin, preruptorin B raises HDL-c and decreases LDL-c. Additionally, praeruptorin B was equivalent to lovastatin in that it dramatically decreased the levels of TC and TG in the liver when compared to mice administered with a vehicle. According to staining results, mice treated with prateruptorin B showed reduced buildup of lipids than mice treated with vehicle. In rats fed a high-fat diet, praeruptorin B dramatically lowers increased fasting blood glucose and insulin levels [1].
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| References |
[1]. Zu-Guo Zheng, et al. Praeruptorin B improves diet-induced hyperlipidemia and alleviates insulin resistance via regulating SREBP signaling pathway. RSC Adv., 2018, 8, 354–366
[2]. Liu X, et al. The Inhibition of UDP-Glucuronosyltransferase (UGT) Isoforms by Praeruptorin A and B. Phytother Res. 2016 Nov;30(11):1872-1878 |
| Molecular Formula |
C24H26O7
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|---|---|
| Molecular Weight |
426.4590
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| CAS # |
73069-28-0
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| SMILES |
O1C2C([H])=C([H])C3C([H])=C([H])C(=O)OC=3C=2C([H])(C([H])(C1(C([H])([H])[H])C([H])([H])[H])OC(/C(=C(/[H])\C([H])([H])[H])/C([H])([H])[H])=O)OC(/C(=C(/[H])\C([H])([H])[H])/C([H])([H])[H])=O
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~25 mg/mL (~58.62 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (5.86 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (5.86 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.3449 mL | 11.7244 mL | 23.4489 mL | |
| 5 mM | 0.4690 mL | 2.3449 mL | 4.6898 mL | |
| 10 mM | 0.2345 mL | 1.1724 mL | 2.3449 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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