| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| 10mg |
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| 25mg |
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| 500mg | |||
| Other Sizes |
| Targets |
IC50: Phytoecdysone
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|---|---|
| ln Vivo |
In the Calliphora bioassay for moulting hormones, Podcedysone B shows about one‑fifth of the activity of crustedysone. This relatively high biological activity is noteworthy because, unlike all other active compounds reported previously, Podcedysone B lacks a 7‑en‑6‑one grouping. [1]
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| References | |
| Additional Infomation |
Podecdysone B is a plant Podecdysone steroid composed of 5β-cholest-8,14-dien-6-one with five hydroxyl substituents at positions 2, 3, 20, 22, and 25. It is a 2β-hydroxy steroid, 3β-hydroxy steroid, 20-hydroxy steroid, 22-hydroxy steroid, 26-hydroxy steroid, 6-oxo steroid, and plant Podecdysone steroid.
Podcedysone B has molecular formula C₂₇H₄₂O₆, melting point 125‑127 °C, UV absorption λmax (ethanol) 244 nm (ε 13,200), and IR νmax (KBr) 3490, 1705, 1650 cm⁻¹. The mass spectrum shows a peak at m/e 444 (M⁺ – H₂O) and peaks at m/e 99 and 81 attributed to side‑chain fragmentation between C‑20 and C‑22, as with crustedysone. Peaks at m/e 344 and 327 correspond to tetracyclic fragments from this cleavage, and a peak at m/e 300 to C‑17–C‑20 fission. The NMR signals (in [²H₆]pyridine) for C‑18, C‑19, C‑21, C‑26/27 methyl groups are reported (δ 1.35, 1.08, 1.55, 1.43 respectively); in [²H₄]methanol the vinylic C‑15 proton appears as a broad multiplet at δ 5.38, with no other vinyl proton absorption. Podcedysone B may be biosynthesised from crustedysone (another constituent of the extract) by dehydration. It is unlikely to be an artefact formed during work‑up because compounds with the 7,14‑dien‑6‑one structure (expected if dehydration had occurred) were not detected. [1] |
| Molecular Formula |
C27H42O6
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|---|---|
| Molecular Weight |
462.61900
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| Exact Mass |
462.298
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| CAS # |
22612-27-7
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| PubChem CID |
441831
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| Appearance |
White to off-white solid
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| Vapour Pressure |
2.89E-21mmHg at 25°C
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| LogP |
2.803
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| Hydrogen Bond Donor Count |
5
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
33
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| Complexity |
889
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| Defined Atom Stereocenter Count |
8
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| SMILES |
O([H])[C@@](C([H])([H])[H])([C@@]([H])(C([H])([H])C([H])([H])C(C([H])([H])[H])(C([H])([H])[H])O[H])O[H])[C@@]1([H])C([H])([H])C([H])=C2C3C([H])([H])C([C@]4([H])C([H])([H])[C@]([H])([C@]([H])(C([H])([H])[C@]4(C([H])([H])[H])C=3C([H])([H])C([H])([H])[C@]12C([H])([H])[H])O[H])O[H])=O
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| InChi Key |
AEFMTBQZWMUASH-IILZZRPCSA-N
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| InChi Code |
InChI=1S/C27H42O6/c1-24(2,32)10-9-23(31)27(5,33)22-7-6-16-15-12-19(28)18-13-20(29)21(30)14-26(18,4)17(15)8-11-25(16,22)3/h6,18,20-23,29-33H,7-14H2,1-5H3/t18-,20+,21-,22-,23+,25-,26+,27+/m0/s1
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| Chemical Name |
(2S,3R,5R,10S,13R,17S)-2,3-dihydroxy-10,13-dimethyl-17-[(2R,3R)-2,3,6-trihydroxy-6-methylheptan-2-yl]-1,2,3,4,5,7,11,12,16,17-decahydrocyclopenta[a]phenanthren-6-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~216.16 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.40 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (5.40 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (5.40 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1616 mL | 10.8080 mL | 21.6160 mL | |
| 5 mM | 0.4323 mL | 2.1616 mL | 4.3232 mL | |
| 10 mM | 0.2162 mL | 1.0808 mL | 2.1616 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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