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    PluriSIn-1 (NSC 14613)
    PluriSIn-1 (NSC 14613)

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    This product is for research use only, not for human use. We do not sell to patients.
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    InvivoChem Cat #: V0863
    CAS #: 91396-88-2Purity ≥98%

    Description: PluriSIn-1 (PluriSln 1; NSC-14613; NSC14613; NSC 14613) is a pluripotent cell-specific inhibitor with important biological activity. It is also a novel and potent inhibitor of the stearoyl-coA desaturase 1 (SCD1), which is able to selectively eliminate hPSCs. 

    References: Cell Stem Cell. 2013 Feb 7;12(2):167-79.

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    Molecular Weight (MW)213.24
    FormulaC12H11N3O
    CAS No.91396-88-2
    Storage-20℃ for 3 years in powder form
    -80℃ for 2 years in solvent
    Solubility (In vitro)DMSO: 43 mg/mL (201.7 mM)
    Water: <1 mg/mL
    Ethanol: <1 mg/mL
    Other infoChemical Name: N'-phenylisonicotinohydrazide
    InChi Key: HUDWXDLBWRHCKO-UHFFFAOYSA-N
    InChi Code: InChI=1S/C12H11N3O/c16-12(10-6-8-13-9-7-10)15-14-11-4-2-1-3-5-11/h1-9,14H,(H,15,16)
    SMILES Code: O=C(NNC1=CC=CC=C1)C2=CC=NC=C2
    SynonymsNSC 14613; PluriSln #1; NSC-14613; NSC14613; PluriSln-1 


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    In Vitro

    In vitro activity: PluriSIns-1 has a robust, rapid, and selective cytotoxic effect toward hPSCs. Apoptosis is the central cell death mechanism activated by PluriSIn #1. PluriSIn #1 leads to ER stress in hPSCs. PluriSIn #1 (20 μM) induces ~30% decrease in protein synthesis in hPSCs. PluriSIns-1 exposure induces a ~65% decrease in stearoyl-coA desaturase (SCD1) activity in hPSCs. PluriSIns-1 (20 μM) prevents teratoma gormation from undifferentiated hPSCs PluriSIns also inhibits mPSCs and hinders mouse embryonic development.


    Kinase Assay: Cells are plated in 6-well plates at a density of 50k to 100k cells per well. 24 h later, 20 μM PluriSIns-1 or 0.2% DMSO-control are added to the cells. After 12 h of incubation at 37 ℃, 5% CO2, the old medium is removed, cells are washed with PBS, and new medium containing 2.3 μM of 0.75 UCi [1-14C] Stearic Acid is added. The cells are incubated for up to 4 h at 37 ℃, 5% CO2. After the incubation period, the medium is discarded and the cells are washed 3 times with 2 mL of PBS. 2 mL of the mixture n-hexane: isopropanol (3:2 v:v) are added, and the cells are incubated for 30 min at 37 ℃, 5% CO2. 2 mL Folch solution (chloroform: methanol,2:1,v:v) are subsequently added. The liquid is transferred to tubes for phase partition by adding 1 mL water. The lower organic phase is evaporated and used for lipid saponification and TLC separation of the free [1-14C] Stearic Acid (substrate) and [1-14C] Oleic Acid (formed product). Lipids extracted from the cells are applied to TLC plates previously immersed in 10% NO3 Ag and activated at 120℃x60 min. Unlabeled stearic and oleic acid are added to each application point as carriers and as internal standards for identification. The plates are run with a solvent mixture of Chloroform:MeOH:AcH:DDW (90:8:1:0.8). The free fatty acids are detected by U.V. after spraying the TLC with a 2',7',dichlorofluorescein solution. The spots corresponding to stearic and oleic acid are scraped and the radioactivity counted in a sc intillating counter. SCD1 desaturase activity is calculated from the percent conversion of substrate to product and the conversion to pmol/min/106 cells.  


    Cell Assay: Relative cell numbers are determined by fixating the cells with 0.5% glutardialdehyde and staining with methylene blue dissolved in 0.1 M boric acid (pH 8.5). Color extraction is performed using 0.1 M hydrochloric acid, and the staining (which is proportional to cell number) is quantitated by measuring absorbance at 650 NM.

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    References

    Cell Stem Cell. 2013 Feb 7;12(2):167-79.


    These protocols are for reference only. InvivoChem does not independently validate these methods.

    PluriSIn-1 (NSC 14613)

    Identification of Pluripotent-Specific Inhibitors. Cell Stem Cell. 2013 Feb 7;12(2):167-79. 
     

    PluriSIn-1 (NSC 14613)

    PluriSIn #1 Induces ER Stress, Protein Synthesis Attenuation, and Apoptosis in hPSCs. Cell Stem Cell. 2013 Feb 7;12(2):167-79. 
     

    PluriSIn-1 (NSC 14613)

    Inhibition of SCD1 Activity Underlies the Cellular Response of hPSCs to PluriSIn #1. Cell Stem Cell. 2013 Feb 7;12(2):167-79. 
     

    PluriSIn-1 (NSC 14613)

    PluriSIns Inhibit Mouse Pluripotent Stem Cells and Hinder Mouse Embryonic Development. Cell Stem Cell. 2013 Feb 7;12(2):167-79. 
     

    PluriSIn-1 (NSC 14613)

    PluriSIn #1 Prevents Teratoma Formation from Undifferentiated hPSCs. Cell Stem Cell.2013 Feb 7;12(2):167-79. 
     

    PluriSIn-1 (NSC 14613)

    A Model of PluriSIn #1 Mechanism of Action. Cell Stem Cell. 2013 Feb 7;12(2):167-79. 


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