Piperoxan hydrochloride (Benodaine hydrochloride)

Alias: F 933 hydrochloride; Piperoxan HCl; benodaine; Piperoxane hydrochloride; F933; F-933
Cat No.:V0047 Purity: ≥98%
Piperoxan hydrochloride(also known as PiperoxanHCl, or benodaine) is a novel, selective and potent α2adrenoceptor antagonist.
Piperoxan hydrochloride (Benodaine hydrochloride) Chemical Structure CAS No.: 135-87-5
Product category: Adrenergic Receptor
This product is for research use only, not for human use. We do not sell to patients.
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Other Forms of Piperoxan hydrochloride (Benodaine hydrochloride):

  • Piperoxan HCl
Official Supplier of:
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Product Description

Piperoxan hydrochloride(also known as Piperoxan HCl, or benodaine) is a novel, selective and potent α2 adrenoceptor antagonist. Moreover, it was the original antihistamine.

Biological Activity I Assay Protocols (From Reference)
Targets
α2 adrenoceptor
ln Vitro
In the event that the medulla is superfused with α2 adrenoceptor antagonist Piperoxane (50 μM; 5 min) and the pons is filled with artificial cerebrospinal fluid (ACSF), three inactive preparations exhibit rhythmic phrenic bursts at a low frequency (2-4 c/min). Meanwhile, the phrenic burst frequency of the twelve active preparations increases significantly during the final 3 min of Piperoxane applications (163±12% of the preceding mean frequency). When the preparations are superfused with either ACSF (n = 8) or the α2 adrenoceptor antagonist Piperoxane (50 μM; PIP-ACSF; n = 5), the effects of NA applications (25 μM; 5 min) are compared. The frequency of phrenic bursts is greatly increased by applying NA, either by itself (NA-ACSF) or in combination with Piperoxane (PIP-ACSF+NA). But piperoxane potentiates a phrenic burst frequency increase by blocking medullary α2 adrenoceptors. In the fifth minute of NA applications, the phrenic burst frequency increased to 171±11% of the mean control value when ACSF is applied alone, and 234±21% of the mean control value when PIP-ACSF is applied in control condition[1].
Cell Assay
The brain stems and cervical spinal cords of the mouse neonates (P0-P3) are removed and placed ventral sides up in a 2 mL chamber superfused with artificial cerebrospinal fluid (ACSF) at 27±0.25°C (mean±SD), renewed at a rate of 2 mL/min. The mice are then given ether anesthesia and decerebrated. By bubbling carbogene (95% O2-5% CO2), the ACSF, which contains (in mM) 129 NaCl, 3.35 KCl, 1.26 CaCl2, 1.15 MgCl2, 21 NaHCO3, 0.58 NaH2PO4, and 30 glucose, is oxygenated and equilibrated (pH 7.4 at 27°C). In the pharmaceutical tests, this is swapped out for an additional ACSF containing bioreactive materials: either α2 adrenoceptor antagonists, such as piperoxane at 50 μM (PIP-ACSF) or yohimbine at 50 μM (YO-ACSF), or noradrenaline at 25 μM (NA-ACSF). In certain experiments, a solution of either ACSF or NA (1 mM) is pressure-expelled from the A5 nucleus after a patch-clamp microelectrode with a diameter of 1 μm is lowered into the ventral pons. For a two-second pressure pulse, the estimated ejected volume is 20 nL[1].
Animal Protocol
Mice: The mice used are male Balb-C mice that weigh 20–25 g. Only slightly opposes the antinociceptive effect of (-)-isoprenaline in mice pretreated with the α-adrenoceptor antagonist Piperoxan or naloxone, both at a dose of 3×10-5 mol /kg s.c. given 15 min before the acetic acid. The antagonists in question yield dose-ratios of 1.45 and 1.7.
References

[1]. Nasal trigeminal inputs release the A5 inhibition received by the respiratory rhythm generator of the mouse neonate. J Neurophysiol. 2004 Feb;91(2):746-58.

[2]. The antinociceptive action of some beta-adrenoceptor agonists in mice. Br J Pharmacol. 1986 Jul;88(3):515-21.

These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C14H20CLNO2
Molecular Weight
269.769
Exact Mass
269.12
Elemental Analysis
C, 62.33; H, 7.47; Cl, 13.14; N, 5.19; O, 11.86
CAS #
135-87-5
Appearance
Solid powder
SMILES
C1CCN(CC1)CC2COC3=CC=CC=C3O2.Cl
InChi Key
BITRJBQGQMGGQI-UHFFFAOYSA-N
InChi Code
InChI=1S/C14H19NO2.ClH/c1-4-8-15(9-5-1)10-12-11-16-13-6-2-3-7-14(13)17-12;/h2-3,6-7,12H,1,4-5,8-11H2;1H
Chemical Name
1-(2,3-dihydro-1,4-benzodioxin-3-ylmethyl)piperidine;hydrochloride
Synonyms
F 933 hydrochloride; Piperoxan HCl; benodaine; Piperoxane hydrochloride; F933; F-933
HS Tariff Code
2934.99.9001
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
DMSO: ~54 mg/mL (~200.2 mM)
Water: ~54 mg/mL
Ethanol: ~40 mg/mL
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 3.7069 mL 18.5343 mL 37.0686 mL
5 mM 0.7414 mL 3.7069 mL 7.4137 mL
10 mM 0.3707 mL 1.8534 mL 3.7069 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

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