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    Piperine
    Piperine

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    This product is for research use only, not for human use. We do not sell to patients.
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    InvivoChem Cat #: V0809
    CAS #: 94-62-2Purity ≥98%

    Description: Piperine (1-Piperoylpiperidine; Bioperine; 1-Piperoyl piperidine), a natural alkaloid found in Piper nigrum L, is an alkaloid accounting for the pungency of black pepper and long pepper, which has been used in some forms of traditional medicine and as an insecticide. It inhibits P-glycoprotein and CYP3A4 activities with an IC50 value of 61.94±0.054 μg/mL in HeLa cell.  

    References: J Pharmacol Exp Ther. 2002 Aug;302(2):645-50; J Ethnopharmacol. 2004 Feb;90(2-3):339-46.

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    FormulaC17H19NO3
    CAS No.94-62-2
    Storage-20℃ for 3 years in powder form
    -80℃ for 2 years in solvent
    Solubility (In vitro)DMSO: 57 mg/mL (199.8 mM)
    Water: <1 mg/mL
    Ethanol: 57 mg/mL (199.8 mM)
    Solubility (In vivo)O=C(N1CCCCC1)/C=C/C=C/C2=CC=C(OCO3)C3=C2
    SynonymsPiperine; 1-Piperoylpiperidine; Bioperine; 1-Piperoyl piperidine


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    In Vitro

    In vitro activity: Piperine (1-Piperoylpiperidine) is the alkaloid responsible for the pungency of black pepper and long pepper, along with chavicine (an isomer of piperine). It has also been used in some forms of traditional medicine and as an insecticide. Piperine has been found to inhibit human CYP3A4 and P-glycoprotein, enzymes important for the metabolism and transport of xenobiotics and metabolites. Piperine is found to be cytotoxic towards DLA and EAC cells at a concentration of 250 μ g/ml.


    Cell Assay: Standard solution is prepared by dissolving 10 mg of piperine in 100 mL of methanol. The MTT assay is carried out to measure cell viability. Ten thousand cells in 100 μL of DMEM media are seeded in the wells of a 96-well plate. After 24 h, existing media is removed and 100 μL of various concentrations of piperine (20–100 μg/mL) are added and incubated for 48 h at 37 °C in a CO2 incubator. Control cells are supplemented with 0.05 % DMSO vehicle. At the 48th hour of incubation, MTT (10 μL of 5 mg/mL) is added to the plate. The contents of the plate are pipetted out carefully, the formazan crystals formed are dissolved in 100 μL of DMSO, and the absorbance is measured at 550 nm in a microplate reader.

    In VivoPiperine (1.14 mg/dose/animal) could inhibit the solid tumor development in mice induced with DLA cells and increase the life span of mice bearing Ehrlich ascites carcinoma tumor. LD50: Mice 15.1mg/kg (i.v.), 43mg/kg (i.p.), 200mg/kg (s.c.), 330mg/kg (i.g.); Rats 33.5mg/kg (i.p.), 514mg/kg (i.g.)
    Animal modelMice and rats
    Formulation & DosageMice 15.1mg/kg (i.v.), 43mg/kg (i.p.), 200mg/kg (s.c.), 330mg/kg (i.g.); Rats 33.5mg/kg (i.p.), 514mg/kg (i.g.)
    References

    J Pharmacol Exp Ther. 2002 Aug;302(2):645-50; J Ethnopharmacol. 2004 Feb;90(2-3):339-46; Toxicol Lett. 1983 May;16(3-4):351-9.


    These protocols are for reference only. InvivoChem does not independently validate these methods.

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