Pioglitazone HCl

Alias: AD-4833, U-72107E; Pioglitazone Hydrochloride; Pioglitazone HCl; Actos;U-72107A;AD-4833; pioglitazone; pioglitazone hydrochloride; U 72107A; U72,107A;U-72107A; AD 4833;
Cat No.:V0820 Purity: ≥98%
Pioglitazone HCl (also known as Actos;U-72107A;AD-4833; AD4833, U-72107E), the hydrochloride salt of Pioglitazone which is approved and thiazolidinedione-based anti-diabetic drug, is a selective PPARϒ agonist with hypoglycemic activity.
Pioglitazone HCl Chemical Structure CAS No.: 112529-15-4
Product category: P450 (e.g. CYP)
This product is for research use only, not for human use. We do not sell to patients.
Size Price Stock Qty
250mg
500mg
1g
2g
Other Sizes

Other Forms of Pioglitazone HCl:

  • Pioglitazone
Official Supplier of:
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Purity & Quality Control Documentation

Purity: ≥98%

Product Description

Pioglitazone HCl (also known as Actos; U-72107A; AD-4833; AD4833, U-72107E), the hydrochloride salt of Pioglitazone which is approved and thiazolidinedione-based anti-diabetic drug, is a selective PPARϒ agonist with hypoglycemic activity. It inhibits PPARϒ with EC50s of 0.93 and 0.99 μM for human and mouse PPARγ, respectively. It is used to treat diabetes.

Biological Activity I Assay Protocols (From Reference)
ln Vitro
Pioglitazone added to the AGEs culture media totally reverses the effects of AGEs-induced beta cell necrosis. Moreover, pioglitazone totally stopped any increase in caspase-3 activation brought on by AGEs, bringing caspase-3 activity back to levels comparable to those of control cells. AG can, as anticipated, mitigate the reduced viability caused by AGEs[2].
ln Vivo
In ob/ob and adipo-/- ob/ob mice, serum-free fatty acid and triglyceride levels, as well as adipocyte sizes, remain unaltered following 10 mg/kg Pioglitazone administration, but are markedly decreased to a comparable extent following 30 mg/kg Pioglitazone. Additionally, after 10 mg/kg of pioglitazone, the expressions of TNFα and resistin in the adipose tissues of ob/ob and adipo-/- ob/ob mice remain unchanged, but after 30 mg/kg of pioglitazone, they drop. As a result, adiponectin may act independently in skeletal muscle and dependently in the liver during pioglitazone-induced improvement of insulin resistance and diabetes[3]. Treatment with pomiglitazone (10 mg/kg per d) effectively reduces heart hypertrophy and body weight loss. Treatment with pioglitazone dramatically lowers increased serum glucose levels and improves the dyslipidemia that is linked with it. Moreover, D rats' serum creatinine levels are marginally but significantly higher than those of their N controls (P <0.05). On the other hand, the diabetic nephropathic (DN) group shows a significant renal impairment (P<0.05). Furthermore, compared to both N and D rats, DN rats have the highest serum activity of CK-MB (P<0.05). Both increased serum levels of creatinine and creatine kinase-MB (CK-MB) can be reduced by pioglitazone[4].
Animal Protocol
Mice: 10 mg/kg Pioglitazone HCl or vehicle (0.25% carboxymethylcellulose) is adnimistered to ob/ob and adipo-/- ob/ob mice by oral gavage once daily for 14 consecutive days. 30 mg/kg Pioglitazone or vehicle is also adnimistered to ob/ob and adipo-/- ob/ob mice by oral gavage once daily for 14 consecutive days.
Rats: Male Wistar albino rats (weighing 250±20 g) are ued.Rats that achieved serum glucose level ≥250 mg/dL and serum creatinine level ≥1.5 mg/dL are divided into 2 groups (n=10 per each group): diabetic nephropathic (DN) group in which rats received an equal amount of vehicle (0.5% carboxy methyl cellulose) and Pioglitazone-treated (DN+Pio) group in which rats treated with Pioglitazone. Pioglitazone (10 mg/kg BW) is given orally by gastric gavage, once daily, for 4 weeks.
Mice and rats
References
[1]. Kuwabara K, et al. A novel selective peroxisome proliferator-activated receptor alpha agonist, 2-methyl-c-5-[4-[5-methyl-2-(4-methylphenyl)-4-oxazolyl]butyl]-1,3-dioxane-r-2-carboxylic acid (NS-220), potently decreases plasma triglyceride and glucose leve
[2]. Puddu A, et al. Pioglitazone attenuates the detrimental effects of advanced glycation end-products in the pancreatic beta cell line HIT-T15. Regul Pept. 2012 Aug 20;177(1-3):79-84.
[3]. Kubota N, et al. Pioglitazone ameliorates insulin resistance and diabetes by both adiponectin-dependent and -independent pathways. J Biol Chem. 2006 Mar 31;281(13):8748-55.
[4]. Elrashidy RA, et al. Pioglitazone attenuates cardiac fibrosis and hypertrophy in a rat model of diabetic nephropathy. J Cardiovasc Pharmacol Ther. 2012 Sep;17(3):324-33
These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C19H20N2O3S.HCL
Molecular Weight
392.9
CAS #
112529-15-4
SMILES
Cl[H].S1C(N([H])C(C1([H])C([H])([H])C1C([H])=C([H])C(=C([H])C=1[H])OC([H])([H])C([H])([H])C1C([H])=C([H])C(=C([H])N=1)C([H])([H])C([H])([H])[H])=O)=O
Synonyms
AD-4833, U-72107E; Pioglitazone Hydrochloride; Pioglitazone HCl; Actos;U-72107A;AD-4833; pioglitazone; pioglitazone hydrochloride; U 72107A; U72,107A;U-72107A; AD 4833;
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
DMSO: 79 mg/mL (201.1 mM)
Water:<1 mg/mL
Ethanol: 4 mg/mL (10.2 mM)
Solubility (In Vivo)

Chemical Name: 5-[[4-[2-(5-ethylpyridin-2-yl)ethoxy]phenyl]methyl]-1,3-thiazolidine-2,4-dione;hydrochloride

InChi Key: GHUUBYQTCDQWRA-UHFFFAOYSA-N

InChi Code: InChI=1S/C19H20N2O3S.ClH/c1-2-13-3-6-15(20-12-13)9-10-24-16-7-4-14(5-8-16)11-17-18(22)21-19(23)25-17;/h3-8,12,17H,2,9-11H2,1H3,(H,21,22,23);1H

SMILES Code: CCC1=CN=C(C=C1)CCOC2=CC=C(C=C2)CC3C(=O)NC(=O)S3.Cl

 (Please use freshly prepared in vivo formulations for optimal results.)
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 2.5452 mL 12.7259 mL 25.4518 mL
5 mM 0.5090 mL 2.5452 mL 5.0904 mL
10 mM 0.2545 mL 1.2726 mL 2.5452 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

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Note: Chemical formula is case sensitive: C12H18N3O4  c12h18n3o4
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In vivo Formulation Calculator (Clear solution)
Step 1: Enter information below (Recommended: An additional animal to make allowance for loss during the experiment)
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Calculation results

Working concentration mg/mL;

Method for preparing DMSO stock solution mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
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