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Purity: ≥98%
PACAP 1-38 is a highly potent agonist of the PACAP (Pituitary adenylate cyclase-activating polypeptide) receptor with Kd of 100 pM. The ADCYAP1 gene in humans encodes the protein PACAP. PACAP and vasoactive intestinal peptide are comparable. It stimulates cells that resemble enterochromaffin, among other things. It binds to both the PACAP receptor and the vasoactive intestinal peptide receptor. The small peptide PACAP 1-38 promotes phagocytosis and adenylate cyclase (AC). Strong, long-lasting, and effective stimulatory effects on sympathetic neuronal NPY and catecholamine production are exhibited by PACAP 1-38. By inhibiting p38 MAPK and NFκB translocation via both PAC1 and VPAC1 receptors, PACAP 1-38 significantly prevents damage to cultured renal proximal tubule cells caused by myeloma light chains. This is achieved through suppression of pro-inflammatory cytokine production.
| Targets |
PAC1 ( Ki = 1.1 nM ); PAC1s ( Ki = 1.7 nM ); PAC1vs ( Ki = 121 nM )
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| Cell Assay |
Human renal proximal tubule cells are cultured for 24 hours at 37°C in DRM-23E medium with 0.5% (vol/vol) FBS after being plated onto 6-well tissue culture plates. Following a prewash with serum-free medium, the cells are cultured with κ-LC (1.5 mg/ml, approximately 50 μM) for three days, both in the presence and absence of different concentrations of transcription factor and kinase inhibitors, as well as PACAP38 or dexamethasone. Trypan blue exclusion assays are used to determine the viability of cells; in all experiments, at least 85% of the cells are still viable. For cytokine assays, culture supernatants are removed and kept at -70°C after being exposed to test substances. The cells are lysed using Sigma Mammalian Cell Lysis Reagents to extract the proteins after the medium is removed, and the cells are then rinsed with ice-cold PBS. Following scraping, lysates are centrifuged at 12,000 g for 10 minutes at 4°C, and a 21-gauge needle is used to shear DNA. Substratum is subsequently collected and utilized in kinase research.
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| References |
| Molecular Formula |
C203H331N63O53S
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| Molecular Weight |
4534.26
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| CAS # |
137061-48-4
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| Related CAS # |
PACAP (1-38), human, ovine, rat TFA; PACAP (1-38) free acid TFA; PACAP (1-38) free acid; 129405-61-4
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| Appearance |
Powder
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| SMILES |
[H]/N=C(/NCCC[C@@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(=O)N)CCCCN)=O)CC(=O)N)=O)CCCCN)=O)C(C)C)=O)CCCN/C(=N/[H])/N)=O)CCC(=O)N)=O)CCCCN)=O)CC1=CC=C(O)C=C1)=O)NC([C@@H](NC(CNC([C@@H](NC([C@H](C(C)C)NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@H](C(C)C)NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@H]([C@H](O)C)NC([C@@H](NC([C@H]([C@H](CC)C)NC(CNC([C@@H](NC([C@@H](NC([C@H](CC1N=CNC=1)N)=O)CO)=O)CC(=O)O)=O)=O)=O)CC1=CC=CC=C1)=O)=O)CC(=O)O)=O)CO)=O)CC1=CC=C(O)C=C1)=O)CO)=O)CCCN/C(=N/[H])/N)=O)CC1=CC=C(O)C=C1)=O)CCCN/C(=N/[H])/N)=O)CCCCN)=O)CCC(=O)N)=O)CCSC)=O)C)=O)=O)CCCCN)=O)CCCCN)=O)CC1=CC=C(O)C=C1)=O)CC(C)C)=O)C)=O)C)=O)=O)CC(C)C)=O)=O)CCCCN)=O)\N
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| Synonyms |
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
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| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
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| Solubility (In Vivo) |
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| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.2205 mL | 1.1027 mL | 2.2054 mL | |
| 5 mM | 0.0441 mL | 0.2205 mL | 0.4411 mL | |
| 10 mM | 0.0221 mL | 0.1103 mL | 0.2205 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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