Oxybutynin chloride

Alias:
Cat No.:V1193 Purity: ≥98%
Oxybutynin chloride (Ditropan;Ditropan XL), the hydrochloride salt of oxybutynin,is a competitive antagonist of muscarinic acetylcholine receptor subtypes M1, M2, and M3used to treat bladder and urinary conditions (i.
Oxybutynin chloride Chemical Structure CAS No.: 1508-65-2
Product category: AChR Receptor
This product is for research use only, not for human use. We do not sell to patients.
Size Price Stock Qty
50mg
100mg
500mg
1g
2g
Other Sizes

Other Forms of Oxybutynin chloride:

  • Oxybutynin
  • (R)-Oxybutynin hydrochloride (Aroxybutynin hydrochloride)
  • Oxybutynin-d11 chloride (oxybutynin d11 (chloride))
  • (R)-Oxybutynin
Official Supplier of:
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Purity & Quality Control Documentation

Purity: ≥98%

Product Description

Oxybutynin chloride (Ditropan; Ditropan XL), the hydrochloride salt of oxybutynin, is a competitive antagonist of muscarinic acetylcholine receptor subtypes M1, M2, and M3 used to treat bladder and urinary conditions (i.e. overactive bladder, urinary and bladder difficulties).

Biological Activity I Assay Protocols (From Reference)
ln Vitro
In coronary artery smooth muscle cells, oxybutynin chloride (0.1, 0.3, 1, 3, 10, 30, 100 μM; 200 ms) inhibits vascular Kv channels in a concentration-dependent manner without causing an anticholinergic effect[1].
ln Vivo
For specific [3H]N-methylscopolamine binding, 0.5 and 2 hours later, oxybutynin chloride (27.2 mg/kg; po; single) shows a 2-fold increase in Kd values[2]. This indicates a significant binding of mouse brain muscarinic receptors.
Cell Assay
Cell Viability Assay[1]
Cell Types: Coronary arterial smooth muscle cells (from Male New Zealand White rabbits)
Tested Concentrations: 10 μM
Incubation Duration: 200 ms
Experimental Results: Rapidly inhibited the Kv current within 2 min and decreased the Kv current by 44% at +60 Mv. Inhibited the Kv current by changing the gating properties of Kv channels.

Cell Viability Assay[1]
Cell Types: Coronary arterial smooth muscle cells (from male New Zealand White rabbits)
Tested Concentrations: 0.1, 0.3, 1, 3, 10, 30, 100 μM
Incubation Duration: 200 ms
Experimental Results: decreased the Kv current amplitude in a concentration-dependent manner, with an IC50 value of 11.51 μM.
Animal Protocol
Animal/Disease Models: Male ddY strain mice (9 to 13weeks old)[2].
Doses: 27.2 mg/kg (76.1 µmol/kg)
Route of Administration: Oral administration; single.
Experimental Results: Significant increased Kd values for specific [3H]NMS binding in mouse bladder with values of 54.7% and 40.6% when at 0.5 and 2 hrs (hours), respectively. Significant increased Kd values for specific [3H]NMS binding in mouse cerebral cortex with values of 120% and 71.2% when at 0.5 and 2 hrs (hours), respectively.
References
[1]. Li H, et al. The anticholinergic drug oxybutynin inhibits voltage-dependent K+ channels in coronary arterial smooth muscle cells. Clin Exp Pharmacol Physiol. 2019 Nov;46(11):1030-1036.
[2]. Oki T, et al. Comparative evaluation of central muscarinic receptor binding activity by oxybutynin, tolterodine and darifenacin used to treat overactive bladder. J Urol. 2007 Feb;177(2):766-70.
These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C22H31NO3.HCL
Molecular Weight
393.95
CAS #
1508-65-2
SMILES
Cl[H].O([H])C(C(=O)OC([H])([H])C#CC([H])([H])N(C([H])([H])C([H])([H])[H])C([H])([H])C([H])([H])[H])(C1C([H])=C([H])C([H])=C([H])C=1[H])C1([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C1([H])[H]
InChi Key
SWIJYDAEGSIQPZ-UHFFFAOYSA-N
InChi Code
InChI=1S/C22H31NO3.ClH/c1-3-23(4-2)17-11-12-18-26-21(24)22(25,19-13-7-5-8-14-19)20-15-9-6-10-16-20;/h5,7-8,13-14,20,25H,3-4,6,9-10,15-18H2,1-2H3;1H
Chemical Name
4-Diethylaminobut-2-ynyl 2-cyclohexyl-2-hydroxy-2-phenylethanoate Hydrochloride
Synonyms

Oxybutynin hydrochloride; Oxybutynin HCl

Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
DMSO: 79 mg/mL (200.5 mM)
Water:4 mg/mL (10.15 mM)
Ethanol:79 mg/mL (200.5 mM)
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 2.5384 mL 12.6920 mL 25.3839 mL
5 mM 0.5077 mL 2.5384 mL 5.0768 mL
10 mM 0.2538 mL 1.2692 mL 2.5384 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

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Method for preparing DMSO stock solution mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

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