| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
| References | |
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| Additional Infomation |
According to reports, Japanese privet and glossy privet contain oleuropein, and relevant data are available for reference.
Oleuropeinic acid was obtained from alperujo after steam treatment (170 °C for 15 min), mild acid hydrolysis (0.5 N HCl), fractionation by Amberlite XAD‑16 chromatography, ultrafiltration through a 1000 Da membrane, and finally size‑exclusion chromatography on a Superdex Peptide HR 10/30 column. It eluted at retention times of 62–68 min, corresponding to oligosaccharides with DP from 4 to 2. Its UV absorption spectrum showed a maximum near 240 nm and a shoulder at 268–274 nm, indicative of secoiridoid glycosides esterified to phenolic compounds. [1] In ESI‑MS (negative mode), characteristic ions were observed at m/z 407 (deprotonated molecule), as well as fragments at m/z 153 and 123 (characteristic of hydroxytyrosol). In some fractions, ions at m/z 569 (loss of hexose, 162 u) and at m/z 705 and 543 were also detected, suggesting additional hexose or hydroxytyrosol moieties. The ¹H and ¹³C NMR spectra (300 and 75 MHz, respectively, in CD₃OD for acetylated fraction E2) were consistent with those previously published for oleuropeinic acid from Ligustrum japonicum. The presence of the ion at m/z 407 in all fractions of the first group, together with the possibility of one or two hexose units linked to the secoiridoid core or to the hydroxytyrosol moiety, allowed speculation on the structure of oleuropeinic acid and several derivatives. [1] |
| Molecular Formula |
C25H30O15
|
|---|---|
| Molecular Weight |
570.496709346771
|
| Exact Mass |
570.158
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| CAS # |
96382-90-0
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| PubChem CID |
146014677
|
| Appearance |
White to off-white solid
|
| LogP |
-1.5
|
| Hydrogen Bond Donor Count |
7
|
| Hydrogen Bond Acceptor Count |
15
|
| Rotatable Bond Count |
12
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| Heavy Atom Count |
40
|
| Complexity |
964
|
| Defined Atom Stereocenter Count |
5
|
| SMILES |
COC(=O)C1=COC(/C(=C/C(=O)O)/C1CC(=O)OCCC2=CC(=C(C=C2)O)O)O[C@H]3[C@@H]([C@H]([C@@H]([C@H](O3)CO)O)O)O
|
| InChi Key |
XJDJODWHDUVAGF-HITGIGKYSA-N
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| InChi Code |
InChI=1S/C25H30O15/c1-36-23(35)14-10-38-24(40-25-22(34)21(33)20(32)17(9-26)39-25)13(7-18(29)30)12(14)8-19(31)37-5-4-11-2-3-15(27)16(28)6-11/h2-3,6-7,10,12,17,20-22,24-28,32-34H,4-5,8-9H2,1H3,(H,29,30)/b13-7+/t12?,17-,20-,21+,22-,24?,25+/m1/s1
|
| Chemical Name |
(2E)-2-[4-[2-[2-(3,4-dihydroxyphenyl)ethoxy]-2-oxoethyl]-5-methoxycarbonyl-2-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-4H-pyran-3-ylidene]acetic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ≥ 100 mg/mL (~175.28 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.43 mg/mL (2.51 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 14.3 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.43 mg/mL (2.51 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 14.3 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 1.43 mg/mL (2.51 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.7528 mL | 8.7642 mL | 17.5285 mL | |
| 5 mM | 0.3506 mL | 1.7528 mL | 3.5057 mL | |
| 10 mM | 0.1753 mL | 0.8764 mL | 1.7528 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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