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| 25mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| 1g |
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Purity: ≥98%
ODQ is a novel, potent and selective inhibitor of soluble guanylyl cyclase (sGC). ODQ and NO have a competitive binding. cGMP is produced by the nitric oxide receptor known as soluble guanylyl cyclase (sGC). Less is known about this second messenger molecule's effects in tumor cells, despite its established roles in cellular physiology. In LNCaP cells, ODQ's activity did not resemble sGC inhibition. Prostate cancer cells are inhibited in their growth and migration, and their ability to die is enhanced by ODQ, all of which occur independently of the compound's impact on GMP levels.
| Targets |
sGC
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|---|---|
| ln Vitro |
Rat cardiomyoblasts are exposed to H2O2 in vitro for 4 hours, which reduces mitochondrial respiration because it produces hydroxyl radicals. ODQ pretreatment of the cells does not prevent this cell damage. Additionally, superoxide anions are not scavenged by ODQ. [1]
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| ln Vivo |
Rats pretreated with ODQ have reduced lung damage, hepatocellular injury, and renal dysfunction brought on by lipoteichoic acid/peptidoglycan or lipopolysaccharide.[1]
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| Cell Assay |
H2O2 is a helpful instrument for researching how various organs are affected by reactive oxygen species. It can easily permeate through cell membranes and, in the presence of transition metals, undergo the Fenton reaction at intracellular locations to transform into the toxic hydroxyl radical. Preincubation of the cells was conducted for two hours at 37°C using ODQ (0.1 mM to 1 mM), saline, or DMSO (media containing 10% DMSO). Following a 4-hour exposure to media or H2O2 (1 mM) at 37°C, the cells' degree of damage is measured. Every experiment is run twice using n = 4 observations.
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| Animal Protocol |
Anesthetized, male Wistar rats
2 mg/kg I.p. |
| References |
| Molecular Formula |
C9H5N3O2
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|---|---|---|
| Molecular Weight |
187.15
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| Exact Mass |
187.04
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| Elemental Analysis |
C, 57.76; H, 2.69; N, 22.45; O, 17.10
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| CAS # |
41443-28-1
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| Related CAS # |
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| Appearance |
Solid powder
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| SMILES |
C1=CC=C2C(=C1)N=CC3=NOC(=O)N23
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| InChi Key |
LZMHWZHOZLVYDL-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C9H5N3O2/c13-9-12-7-4-2-1-3-6(7)10-5-8(12)11-14-9/h1-5H
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| Chemical Name |
[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one
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| Synonyms |
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
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| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 5 mg/mL (26.72 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 50.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.3433 mL | 26.7165 mL | 53.4331 mL | |
| 5 mM | 1.0687 mL | 5.3433 mL | 10.6866 mL | |
| 10 mM | 0.5343 mL | 2.6717 mL | 5.3433 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
| NCT Number | Recruitment | interventions | Conditions | Sponsor/Collaborators | Start Date | Phases |
| NCT05535270 | Completed | Diagnostic Test: Oxford Depression Questionnaire (ODQ) |
Mood Disorders Emotional Blunting |
First Affiliated Hospital of Zhejiang University |
January 1, 2022 | N/A |
Inhibition of cGMP accumulation by ODQ.
ODQ-inhibited migration of LNCaP cells.Br J Pharmacol.2008 Nov;155(6):804-13. |
ODQ-enhanced cell death in LNCaP cells.Br J Pharmacol.2008 Nov;155(6):804-13. |
Caspase-3 activity in normal human prostate epithelial cells (HPrECs) was not increased following ODQ treatment. |
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