| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| 250mg |
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Purity: ≥98%
| ln Vitro |
O4I2 not only stimulates Oct3/4 expression by activating its promoter site, but also stabilizes the Oct3/4 protein in HEK293 cells[1]. O4I2 substantially activates Oct3/4 even at 5 µM after 72 hrs treatment on the translational level[1]. O4I2 (20 µM) accumulates Oct3/4 in NCCIT happened already 2 hours after incubation, but the measurable Sox2 increase emerges much later, implying compound-mediated activation of Oct3/4 might be different between embryonic kidney HEK293 cells and embryonal cancer NCCIT cells [1].
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| ln Vivo |
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| Cell Assay |
Immunofluorescence[1]
Cell Types: HF (human fibroblasts) cells. Tested Concentrations: 5-20 µM. Incubation Duration: 5 days. Experimental Results: Successfully detected co-expression of Oct3/4 and Sox2, as well as Nanog and Klf4. |
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| Animal Protocol |
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| References |
[1]. Cheng X, et al. Ethyl 2-((4-Chlorophenyl)amino)thiazole-4-carboxylate and Derivatives Are Potent Inducers of Oct3/4. J Med Chem. 2015 Aug 13;58(15):5742-5750.
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| Molecular Formula |
C12H11CLN2O2S
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| Molecular Weight |
282.75
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| CAS # |
165682-93-9
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| Related CAS # |
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| SMILES |
ClC1C([H])=C([H])C(=C([H])C=1[H])N([H])C1=NC(C(=O)OC([H])([H])C([H])([H])[H])=C([H])S1
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| InChi Key |
ULUBAPWNHROTEU-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C12H11ClN2O2S/c1-2-17-11(16)10-7-18-12(15-10)14-9-5-3-8(13)4-6-9/h3-7H,2H2,1H3,(H,14,15)
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| Chemical Name |
ethyl 2-((4-chlorophenyl)amino)thiazole-4-carboxylate
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| Synonyms |
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
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| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (8.84 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.5367 mL | 17.6835 mL | 35.3669 mL | |
| 5 mM | 0.7073 mL | 3.5367 mL | 7.0734 mL | |
| 10 mM | 0.3537 mL | 1.7683 mL | 3.5367 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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