| Size | Price | Stock | Qty |
|---|---|---|---|
| 250mg |
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| 500mg |
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| 5g |
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| Other Sizes |
| Targets |
o-Phenanthroline targets metalloproteases by chelating divalent metal ions, particularly iron (Fe2+), zinc, and other divalent metals. It forms a stable red complex with Fe2+ that absorbs maximally at 510 nm. This chelation inhibits zinc-dependent hydrolysis of substrates by metalloproteases.
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|---|---|
| ln Vitro |
o-Phenanthroline scaffold (1,10-phenanthroline, 1 mM) interferes with the autolysis of the body wall of sea cucumbers [2].
o-Phenanthroline acts as a general metalloprotease inhibitor. It prevents the induction of chromosomal aberrations in streptozotocin-treated cells. The compound is effective at concentrations of 1-10 mM and is used to study DNA-protein interactions due to its nuclease activity. |
| ln Vivo |
In vivo activity of o-Phenanthroline is primarily related to its metal chelation properties. It has been employed as a photometric reagent for iron determination. Specific in vivo pharmacological data are not extensively reported in the available literature.
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| Enzyme Assay |
Metal chelation assays are performed by incubating o-Phenanthroline with metal ions and measuring the formation of colored complexes. MMP inhibition assays are conducted using metalloprotease enzymes and peptide substrates. The compound's ability to prevent chromosomal aberrations is assessed in cell-based systems.
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| Cell Assay |
Cell-based assays are conducted to evaluate the compound's ability to prevent chromosomal aberrations in streptozotocin-treated cells. The compound's nuclease activity is studied in DNA-protein interaction assays.
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| Animal Protocol |
In vivo studies are not extensively reported. The compound is primarily used as a research reagent for metal chelation and MMP inhibition studies. Its applications are in analytical chemistry and biochemical research.
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| ADME/Pharmacokinetics |
o-Phenanthroline monohydrate has a molecular weight of 198.23. It is slightly soluble in water (3.3 g/l at room temperature) and fairly soluble in alcohol (540 g/l). Storage: dry, inert atmosphere; shelf-life of two years if stored dry.
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| Toxicity/Toxicokinetics |
o-Phenanthroline is a metal chelator and MMP inhibitor. It should be handled with appropriate personal protective equipment. The compound is for research use only and is not intended for human therapeutic use.
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| References |
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| Additional Infomation |
o-Phenanthroline monohydrate is a white crystalline powder or solid with a slight odor. (NTP, 1992)
o-Phenanthroline monohydrate is a metal chelator used as a photometric reagent for iron, an MMP inhibitor, and a tool for studying DNA-protein interactions. Synonyms include 1,10-Phenanthroline monohydrate. |
| Molecular Formula |
C₁₂H₁₀N₂O
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|---|---|
| Molecular Weight |
198.22
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| Exact Mass |
198.079
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| CAS # |
5144-89-8
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| Related CAS # |
o-Phenanthroline;66-71-7
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| PubChem CID |
21226
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| Appearance |
White to off-white solid powder
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| Density |
1.10
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| Boiling Point |
365.1ºC at 760 mmHg
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| Melting Point |
100-104 °C(lit.)
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| Flash Point |
164.8ºC
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| LogP |
2.718
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
15
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| Complexity |
183
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
PPQJCISYYXZCAE-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C12H8N2.H2O/c1-3-9-5-6-10-4-2-8-14-12(10)11(9)13-7-1;/h1-8H;1H2
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| Chemical Name |
1,10-phenanthroline;hydrate
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| Synonyms |
oPhenanthroline monohydrate; o Phenanthroline monohydrate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~504.49 mM)
H2O : ~2 mg/mL (~10.09 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (12.61 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (12.61 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (12.61 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.0449 mL | 25.2245 mL | 50.4490 mL | |
| 5 mM | 1.0090 mL | 5.0449 mL | 10.0898 mL | |
| 10 mM | 0.5045 mL | 2.5224 mL | 5.0449 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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