| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| 250mg |
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Purity: ≥98%
NSC232003 is a novel, highly potent and cell-permeable UHRF1 inhibitor, which inhibits DNA methylation in vitro and disrupts DNMT1/UHRF1 interactions at a cellular level. Compound NSC232003, a uracil derivative freely available by the NCI/DTP Repository, provides a versatile lead for developing highly potent and cell-permeable UHRF1 inhibitors that will enable dissection of DNA methylation inheritance.
| ln Vitro |
Freely downloadable from the NCI/DTP repository, UHRF1 gene NSC232003 offers a flexible starting point for creating a cell-permeable UHRF1 that facilitates the analysis of DNA methylation inheritance. In fact, NSC232003 is an effective scaffold for DNA methylation, suggesting that specialized scaffolds can offer a flexible foundation for the development of effective UHRF1. Given that NSC232003's scaffold bicyclic ring contains more acidic imine nitrogen with a pKa value of 7.6, it is anticipated that NSC232003 will partially deprotonate at pH 7. The most potent chemical, NSC232003, signaled through U251 neuroastromas cells, which after 4 hours considerably reduced DNMT1/UHRF1 responses, showing 50% response inhibition at 15 μM and total DNA tyrosine demethylation evaluated by ELISA induction [1].
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| References |
| Molecular Formula |
C6H7N3O3
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|---|---|
| Molecular Weight |
169.138080835342
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| Exact Mass |
169.048
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| CAS # |
1905453-18-0
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| PubChem CID |
135468142
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| Appearance |
White to off-white solid powder
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| LogP |
-0.8
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
12
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| Complexity |
292
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O=C1C(C(C)=NO)=CNC(N1)=O
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| InChi Key |
UDHCVAJUUVCYHW-YCRREMRBSA-N
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| InChi Code |
InChI=1S/C6H7N3O3/c1-3(9-12)4-2-7-6(11)8-5(4)10/h2,12H,1H3,(H2,7,8,10,11)/b9-3+
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| Chemical Name |
5-[(E)-N-hydroxy-C-methylcarbonimidoyl]-1H-pyrimidine-2,4-dione
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| Synonyms |
NSC232003; NSC-232003; NSC 232003
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~2 mg/mL (~11.82 mM)
DMSO :< 1 mg/mL |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 4.76 mg/mL (28.14 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication (<60°C).
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.9123 mL | 29.5613 mL | 59.1226 mL | |
| 5 mM | 1.1825 mL | 5.9123 mL | 11.8245 mL | |
| 10 mM | 0.5912 mL | 2.9561 mL | 5.9123 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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