| Size | Price | Stock | Qty |
|---|---|---|---|
| 500mg |
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| Other Sizes |
| Targets |
NOV-002 is a mimetic of oxidized glutathione (GSSG). It is a glutathione derivative and an organic disulfide. The compound has a role as an Escherichia coli metabolite and a mouse metabolite. It impacts cellular redox balance.
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|---|---|
| ln Vitro |
Red blood cells' glutathione oxidation concentration can be raised by adding (2,3-dimethylevo-1,4-ethoquinone) DMNQ [1]. Comparing the stimulation of H2O2 in sickle versus healthy red blood cells [1].
In vitro, NOV-002 functions as a glutathione disulfide mimetic. It impacts cellular redox balance, which is critical to the maintenance of cell viability. The compound is an antioxidant that can react with free radicals and reduce cellular oxidative damage. It also has anti-inflammatory effects. |
| ln Vivo |
NOV-002 is a mechanistically novel agent with potential for ameliorating hematologic toxicity and enhancing efficacy when used in combination with standard chemotherapy to treat cancer patients. The compound has been studied for its ability to modulate redox balance and protect against chemotherapy-induced toxicity.
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| Enzyme Assay |
NOV-002 can be studied in vitro using cell-based assays that measure cellular redox status. Cells are treated with the compound, and glutathione levels (reduced and oxidized) are measured. Oxidative stress markers (ROS, lipid peroxidation) are assessed. The compound's ability to modulate redox-sensitive signaling pathways is evaluated. Cytoprotective effects against chemotherapy-induced toxicity can be assessed.
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| Cell Assay |
Cell-based assays for NOV-002 involve treating various cell lines with the compound and assessing its effects on cell viability, oxidative stress, and redox balance. Glutathione levels are measured using enzymatic assays or HPLC. ROS generation is detected using fluorescent probes. The compound's ability to protect cells from oxidative damage is assessed in models of oxidative stress. Anti-inflammatory effects are evaluated by measuring cytokine production.
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| Animal Protocol |
In vivo animal studies for NOV-002 typically involve administering the compound to tumor-bearing mice in combination with chemotherapy. The compound's ability to reduce chemotherapy-induced hematologic toxicity and enhance antitumor efficacy is assessed. Blood cell counts are monitored. Tumor growth inhibition is measured. Survival is assessed. The compound's effects on redox balance and inflammation in vivo are evaluated.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of NOV-002 are related to its role as a glutathione disulfide mimetic. The compound is a small molecule that can be administered systemically. It is metabolized to glutathione and other related compounds. Its half-life is relatively short. The compound's effects on cellular redox balance are transient. Excretion occurs through renal pathways.
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| Toxicity/Toxicokinetics |
Toxicological profile of NOV-002 is favorable. The compound is a mimetic of an endogenous molecule (GSSG) and is generally well-tolerated. It has been studied in clinical trials and shown to have a good safety profile. Common side effects are mild. The compound's antioxidant properties may protect against chemotherapy-induced toxicity. No significant off-target toxicity has been reported.
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| References |
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| Additional Infomation |
Glutathione disulfide (GSSG) is an organic disulfide and a derivative of glutathione. It is a metabolite in both E. coli and mice. It is the conjugate acid of glutathione disulfide (2-). Glutathione disulfide is a glutathione dimer formed by the oxidation of the cysteine sulfhydryl side chain via disulfide bonds. GSSG is a metabolite found or produced in E. coli (K12 strain, MG1655 strain). Oxidized glutathione has also been reported in Drosophila melanogaster, Enterococcus faecalis, and other organisms with relevant data. Oxidized glutathione is the disulfide form of glutathione (GSH) and possesses potential protective activity. GSSG can be reduced to GSH by glutathione reductase. GSSG and GSH jointly participate in various redox reactions, such as detoxification reactions involving harmful substances and free radicals, and reactions that prevent oxidative damage to erythrocytes. Adding glutathione disulfide to ocular infusion solutions can have a beneficial effect on the redox state of intracellular glutathione, thereby protecting the integrity and barrier function of corneal endothelial cells. Glutathione disulfide is a glutathione dimer formed by disulfide bonds through the sulfhydryl side chain of cysteine during oxidation. See also: ... (See more...)
NOV-002 is a mechanistically novel agent with potential for ameliorating hematologic toxicity and enhancing efficacy when used in combination with standard chemotherapy to treat cancer patients. The compound has been evaluated in clinical trials as an adjunct to chemotherapy. It is a mimetic of oxidized glutathione and is also known as oxiglutatione. |
| Molecular Formula |
C20H32N6O12S2
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|---|---|
| Molecular Weight |
612.63
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| Exact Mass |
612.151
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| CAS # |
27025-41-8
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| Related CAS # |
Glutathione oxidized-13C4,15N2;1416898-83-3;Glutathione oxidized disodium;103239-24-3
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| PubChem CID |
65359
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| Appearance |
White to off-white solid powder
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| Density |
1.7±0.1 g/cm3
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| Boiling Point |
976.3±75.0 °C at 760 mmHg
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| Melting Point |
178 °C (dec.)(lit.)
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| Flash Point |
544.3±37.1 °C
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| Vapour Pressure |
0.0±0.6 mmHg at 25°C
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| Index of Refraction |
1.677
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| LogP |
1.91
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| Hydrogen Bond Donor Count |
10
|
| Hydrogen Bond Acceptor Count |
16
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| Rotatable Bond Count |
21
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| Heavy Atom Count |
40
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| Complexity |
879
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| Defined Atom Stereocenter Count |
4
|
| SMILES |
C(CC(=O)N[C@@H](CSSC[C@@H](C(=O)NCC(=O)O)NC(=O)CC[C@@H](C(=O)O)N)C(=O)NCC(=O)O)[C@@H](C(=O)O)N
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| InChi Key |
YPZRWBKMTBYPTK-BJDJZHNGSA-N
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| InChi Code |
InChI=1S/C20H32N6O12S2/c21-9(19(35)36)1-3-13(27)25-11(17(33)23-5-15(29)30)7-39-40-8-12(18(34)24-6-16(31)32)26-14(28)4-2-10(22)20(37)38/h9-12H,1-8,21-22H2,(H,23,33)(H,24,34)(H,25,27)(H,26,28)(H,29,30)(H,31,32)(H,35,36)(H,37,38)/t9-,10-,11-,12-/m0/s1
|
| Chemical Name |
(2S)-2-amino-5-[[(2R)-3-[[(2R)-2-[[(4S)-4-amino-4-carboxybutanoyl]amino]-3-(carboxymethylamino)-3-oxopropyl]disulfanyl]-1-(carboxymethylamino)-1-oxopropan-2-yl]amino]-5-oxopentanoic acid
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| Synonyms |
NOV 002; NOV-002; NOV002
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~250 mg/mL (~408.08 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (163.23 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.6323 mL | 8.1615 mL | 16.3231 mL | |
| 5 mM | 0.3265 mL | 1.6323 mL | 3.2646 mL | |
| 10 mM | 0.1632 mL | 0.8162 mL | 1.6323 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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