| Size | Price | Stock | Qty |
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| 50g |
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| 100g |
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| Other Sizes |
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Purity: ≥98%
| ln Vitro |
Nonivamide is a synthetic capsaicin derivative that has strong antifouling properties. For capsaicin, the 4d-EC50 values for Pseudomonas putida, Lake Erie bacteria, and Vibrio natrigeni were 5.5±0.5 mg/L, 23±2 mg/L, and 6.9±0.2 mg/L and 15.6±0.4 mg/L, and V. parahaemolyticus, respectively, in static toxicity experiments. The 1 mg/L Nonivamide treatment group showed significant growth inhibition for 4 days (p<0.01), with an EC50 value of 5.1 mg/L for the 4 day-EC50[1]. Treatment with nonivamide releases calcium from the endoplasmic reticulum (ER) and modifies transcription of growth arrest and DNA damage-induced transcript 3 (GADD153), GADD45α, GRP78/BiP, ATF3, CCND1, and CCNG2) in a way characteristic of usual ER stress. After pretreating cells with 2.5 μM thapsigargin for 5 min, ER calcium flow was measured by adding 2.5 μM Nonivamide. Calcium from ER reserves was released when 2.5 μM Nonivamide was given to TRPV1-overexpressing cells, leading to a notable rise in cytosolic calcium. After 24 hours, treating TRPV1-overexpressing cells with 1 μM Nonivamide led to a roughly 50% reduction in cell viability. Additionally, BEAS-2B cells treated with 100 and 200 μM Nonivamide displayed an increase in GADD153 mRNA and protein expression as well as alterations in the relative amount of EIF2α-P [2]. At every examined concentration, the effects of nonivamide treatment on lipid buildup were identical to those following CAP treatment in terms of reduction. Nonivamide treatment decreased lipid accumulation by 5.34±1.03% (P<0.05) at 0.01 µM and by 10.4±2.47% (P<0.001) at 1 µM in comparison to untreated control cells [3].
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| References |
[1]. Zhou J, et al. Toxic effects of environment-friendly antifoulant Nonivamide on Phaeodactylum tricornutum. Environ Toxicol Chem. 2013 Apr;32(4):802-9.
[2]. Thomas KC, et al. Transient receptor potential vanilloid 1 agonists cause endoplasmic reticulum stress and cell death in human lung cells. J Pharmacol Exp Ther. 2007 Jun;321(3):830-8. [3]. Rohm B, et al. Nonivamide enhances miRNA let-7d expression and decreases adipogenesis PPARγ expression in 3T3-L1 cells. J Cell Biochem. 2015 Jun;116(6):1153-63 |
| Molecular Formula |
C18H27NO3
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|---|---|
| Molecular Weight |
305.4119
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| CAS # |
2444-46-4
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| SMILES |
O=C(C([H])([H])C([H])([H])C([H])([H])C([H])([H])/C(/[H])=C(\[H])/C([H])(C([H])([H])[H])C([H])([H])[H])N([H])C([H])([H])C1C([H])=C([H])C(=C(C=1[H])OC([H])([H])[H])O[H]
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| Synonyms |
Nonivamide
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~340.83 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (8.52 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (8.52 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (8.52 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.2743 mL | 16.3714 mL | 32.7429 mL | |
| 5 mM | 0.6549 mL | 3.2743 mL | 6.5486 mL | |
| 10 mM | 0.3274 mL | 1.6371 mL | 3.2743 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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