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    Nexturastat A (AG-CR1-3901)
    Nexturastat A (AG-CR1-3901)

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    This product is for research use only, not for human use. We do not sell to patients.
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    InvivoChem Cat #: V0270
    CAS #: 1403783-31-2Purity ≥98%

    Description: Nexturastat A (AG-CR1-3901), an aryl urea analog, is a novel, potent and selective inhibitor of histone deacetylase 6 (HDAC6) with potential anticancer activity. It inhibits HDAC6 with an IC50 of 5 nM, and exhibits >190-fold selectivity for HDAC6 over other HDACs. It was developed by structural modifications based on an aryl urea HDACI. 

    References: J Med Chem. 2012 Nov 26;55(22):9891-9.

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    Molecular Weight (MW)341.4
    FormulaC19 H23 N3 O3
    CAS No.1403783-31-2
    Storage-20℃ for 3 years in powder form
    -80℃ for 2 years in solvent
    Solubility (In vitro)DMSO: 68 mg/mL (199.2 mM)
    Water: <1 mg/mL
    Ethanol: 2 mg/mL (5.9 mM)
    Other info

    Chemical Name: 4-((1-butyl-3-phenylureido)methyl)-N-hydroxybenzamide

    InChi Key: JZWXMCPARMXZQV-UHFFFAOYSA-N

    InChi Code: InChI=1S/C19H23N3O3/c1-2-3-13-22(19(24)20-17-7-5-4-6-8-17)14-15-9-11-16(12-10-15)18(23)21-25/h4-12,25H,2-3,13-14H2,1H3,(H,20,24)(H,21,23)
    SMILES Code: O=C(NO)C1=CC=C(CN(CCCC)C(NC2=CC=CC=C2)=O)C=C1 
    SynonymsNexturastat A; AG-CR13901


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    In Vitro

    In vitro activity: In B16 murine melanoma cells, Nexturastat A leads to a dose-dependent increase of acetyl α-tubulin levels without a concomitant elevation of histone H3 acetylation. Moreover, Nexturastat A potently inhibits the growth of B16 melanoma cells with GI50 of 14.3 μM.


    Kinase Assay: HDAC inhibition assays are performed by Reaction Biology Corp. using isolated human, recombinant full2length HDAC1 and -6 from a baculovirus expression system in Sf9 cells. An acetylated fluorogenic peptide,RHKKAc, derived from residues 379-382 of p53 is used as substrate. The reaction buffer is made up of 50 mM Tris-HCl pH 8.0, 127 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 1 mg/mL BSA, and a final concentration of 1% DMSO. Compounds are delivered in DMSO and delivered to enzyme mixture with preincubation of 5-10 min followed by substrate addition and incubation for 2 h at 30°C. Trichostatin A and developer are added to quench the reaction and generate fluorescence, respectively. Dose-response curves are generated starting at 30 μM compound with three-fold serial dilutions to generate a 10-dose plot. IC50 values are then generated from the resulting plots, and the values expressed are the average of duplicate trials ± standard deviation.


    Cell Assay: B16 murine melanoma cells are plated at 5000/well in 96 well flat bottom plates. The following day, media is changed to that containing various concentrations of HDACi or matched DMSO vehicle concentrations diluted in complete medium done in triplicate. Cells are incubated for 48 hours at 37°C and 5% CO2. Density of viable, metabolically active cells is quantified using a standard MTS assay as per manufacturer’s instructions. Briefly, 20μL of reagent are added per well and incubated at 37°C for 3 hours. Absorbances at 490nM are measured spectrophotometrically with background subtraction at 690 nM. All values are then normalized and expressed as a percentage of medium control (100%).

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    Formulation & DosageNA
    References

    J Med Chem. 2012 Nov 26;55(22):9891-9.


    These protocols are for reference only. InvivoChem does not independently validate these methods.

     

    Nexturastat A

    Structure of 1, a urea containing HDACI. Appendage of substitutions to the nitrogen atoms of the urea generate a branched linker motif that may interact with a side cavity at the enzyme surface. J Med Chem. 2012 Nov 26;55(22):9891-9. 
     

    Nexturastat A

    Comparison of acetylation status of tubulin and histone H3 demonstrates HDAC62substrate selectivity of Nexturastat A. J Med Chem. 2012 Nov 26;55(22):9891-9. 


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