| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
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Purity: ≥98%
Nevanimibe HCl (also known as PD-132301 hydrochloride and ATR-101 hydrochloride), the hydrochloride salt of Nevanimibe, is a novel, selective and potent acyl-coenzyme A: cholesterol O-acyltransferase 1 (ACAT1) inhibitor with an EC50 of 9 nM. It inhibits ACAT2 with an EC50of 368 nM.
| ln Vitro |
The frequency of spleen was reduced by 60% when nervanibib hydrochloride (PD-132301 hydrochloride; ATR101 hydrochloride; 3 nM-30 μM) and cholesterol were depleted together. The toxicity was shown to rise in a dose-dependent way, with 3 nM nervanibib at 60 μg. 24 In the presence of cholesterol, all dosages of Nevanimibe (3 nM–30 μM) caused cytotoxicity, while treatment with cholesterol in the absence of Nevanimibe had no effect on cell viability. Influence [1].
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|---|---|
| Cell Assay |
Cytotoxicity assay [1]
Cell Types: H295R and HAC clone 15 (HAC15) Human ACC cell line Tested Concentrations: 3 nM-30 μM Incubation Duration: 24 hrs (hours) Experimental Results: 3 nM-3 μM non-toxic, while 30 μM treatment improved survival diminished by approximately 40% in 24 hrs (hours). |
| References |
| Molecular Formula |
C27H40CLN3O
|
|---|---|
| Molecular Weight |
458.0790
|
| Exact Mass |
457.286
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| CAS # |
133825-81-7
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| Related CAS # |
Nevanimibe;133825-80-6
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| PubChem CID |
131678
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| Appearance |
White to off-white solid powder
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| Boiling Point |
528.1ºC at 760 mmHg
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| Flash Point |
273.2ºC
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| Vapour Pressure |
3.05E-11mmHg at 25°C
|
| LogP |
7.712
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
32
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| Complexity |
543
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
SDOOGTHIDFZUNM-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C27H39N3O.ClH/c1-19(2)23-10-9-11-24(20(3)4)25(23)29-26(31)28-18-27(16-7-8-17-27)21-12-14-22(15-13-21)30(5)6;/h9-15,19-20H,7-8,16-18H2,1-6H3,(H2,28,29,31);1H
|
| Chemical Name |
1-[[1-[4-(dimethylamino)phenyl]cyclopentyl]methyl]-3-[2,6-di(propan-2-yl)phenyl]urea;hydrochloride
|
| Synonyms |
PD-132301 hydrochloride; PD132301 hydrochloride; PD 132301 hydrochloride; ATR-101 hydrochloride;ATR 101 hydrochloride;ATR101 hydrochloride;
|
| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~41.67 mg/mL (~90.97 mM)
H2O : < 0.1 mg/mL |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (4.54 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (4.54 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (4.54 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1830 mL | 10.9151 mL | 21.8302 mL | |
| 5 mM | 0.4366 mL | 2.1830 mL | 4.3660 mL | |
| 10 mM | 0.2183 mL | 1.0915 mL | 2.1830 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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