| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
|
||
| 500mg |
|
| Enzyme Assay |
- For NADP-dependent enzyme cofactor binding assay: Three-dimensional structures of NADP-dependent enzymes were analyzed to identify conserved cofactor binding motifs. NADP disodium salt was used as the cofactor to incubate with purified enzymes, and binding interactions were characterized by structural superimposition and sequence alignment. The stereochemical features of NADP disodium salt binding to the enzyme active site, including hydrogen bonds and hydrophobic contacts, were determined [1]
|
|---|---|
| Cell Assay |
- For intracellular NADP+ detection assay: Cells were transfected with a genetically encoded biosensor plasmid. After transfection, cells were incubated in medium containing NADP disodium salt as the precursor for intracellular NADP+ synthesis. Fluorescence signals from the biosensor were measured using confocal microscopy to quantify intracellular NADP+ levels. The biosensor showed specific response to NADP disodium salt-derived NADP+ without cross-reacting with NAD+ [2]
|
| References | |
| Additional Infomation |
NADP is a metabolite found or produced in Escherichia coli (strains K12 and MG1655). NADP, or nicotinamide adenine dinucleotide phosphate, is a redox coenzyme present in eukaryotic cells and participates in various enzymatic reactions. Nicotinamide adenine dinucleotide phosphate is a coenzyme composed of ribosylnicotinamide 5'-phosphate (NMN) linked to 5'-adenosine 2',5'-bisphosphate (A2P) via a pyrophosphate bond. It acts as an electron carrier in various reactions and is alternately oxidized (NADP+) and reduced (NADPH). (Dorland, 27th edition) - Disodium NADP is the disodium form of nicotinamide adenine dinucleotide phosphate (NADP), a key cofactor in intracellular redox reactions [1][2][3] - The phosphate group of disodium NADP is crucial for its specific binding to NADP-dependent enzymes, which distinguishes it from NAD [3] - Disodium NADP participates in anabolic pathways such as fatty acid synthesis, nucleotide synthesis, and photosynthesis, and participates in cellular antioxidant defense through the glutathione system [3]
|
| Molecular Formula |
C21H26N7NA2O17P3
|
|---|---|
| Molecular Weight |
787.3687
|
| Exact Mass |
787.039
|
| CAS # |
24292-60-2
|
| Related CAS # |
53-57-6 (reduced);604-79-5 (oxidized);53-59-8 (free);1184-16-3 (Na); 100929-71-3 (ammonium); 24294-60-2 (disodium);
|
| PubChem CID |
2734411
|
| Appearance |
White to off-white solid powder
|
| Hydrogen Bond Donor Count |
6
|
| Hydrogen Bond Acceptor Count |
21
|
| Rotatable Bond Count |
12
|
| Heavy Atom Count |
50
|
| Complexity |
1280
|
| Defined Atom Stereocenter Count |
8
|
| SMILES |
C1=CC(=C[N+](=C1)[C@H]2[C@@H]([C@@H]([C@H](O2)COP(=O)([O-])OP(=O)(O)OC[C@@H]3[C@H]([C@H]([C@@H](O3)N4C=NC5=C(N=CN=C54)N)OP(=O)([O-])[O-])O)O)O)C(=O)N.[Na+].[Na+]
|
| InChi Key |
WSDDJLMGYRLUKR-WUEGHLCSSA-L
|
| InChi Code |
InChI=1S/C21H28N7O17P3.2Na/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(44-46(33,34)35)14(30)11(43-21)6-41-48(38,39)45-47(36,37)40-5-10-13(29)15(31)20(42-10)27-3-1-2-9(4-27)18(23)32;;/h1-4,7-8,10-11,13-16,20-21,29-31H,5-6H2,(H7-,22,23,24,25,32,33,34,35,36,37,38,39);;/q;2*+1/p-2/t10-,11-,13-,14-,15-,16-,20-,21-;;/m1../s1
|
| Chemical Name |
disodium;[(2R,3R,4R,5R)-2-(6-aminopurin-9-yl)-5-[[[[(2R,3S,4R,5R)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-oxidophosphoryl]oxy-hydroxyphosphoryl]oxymethyl]-4-hydroxyoxolan-3-yl] phosphate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O : ~250 mg/mL (~316.70 mM)
DMSO : ~3.33 mg/mL (~4.22 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (126.68 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.2701 mL | 6.3503 mL | 12.7005 mL | |
| 5 mM | 0.2540 mL | 1.2701 mL | 2.5401 mL | |
| 10 mM | 0.1270 mL | 0.6350 mL | 1.2701 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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