| Size | Price | Stock | Qty |
|---|---|---|---|
| 250mg |
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| 500mg |
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| 1g |
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| 2g | |||
| Other Sizes |
| Targets |
Human Endogenous Metabolite
N-acetylhistamine targets the Histamine Receptor and is classified as an Endogenous Metabolite. As a metabolite of histamine, it is involved in histidine metabolism and histamine turnover. The compound's role as a biomarker for histamine turnover and anaphylactoid reactions is based on its production and clearance in the body. Its levels reflect the activity of the histamine metabolic pathway. |
|---|---|
| ln Vitro |
N-acetylhistamine is a crucial indicator for anaphylactoid reactions[1].
N-Acetylhistamine does not exhibit significant direct biological activity as a therapeutic agent. Its primary role is as a metabolite of histamine and a biomarker for histidine metabolism. The compound's levels in biological fluids reflect histamine turnover and can be used to monitor allergic reactions and anaphylactoid responses. |
| ln Vivo |
N-Acetylhistamine is a metabolite of histamine that is formed endogenously and excreted in urine. Its levels in biological fluids reflect histamine turnover and can be used as a biomarker for histidine metabolism and anaphylactoid reactions.
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| Enzyme Assay |
In vitro non-cell assays for N-acetylhistamine are not standard, as the compound is primarily used as a biomarker. Its levels in biological samples can be measured using analytical techniques such as HPLC, LC-MS/MS, or ELISA. Standard curves are prepared using authentic standards to quantify the compound in samples.
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| Cell Assay |
In vitro cell-based assays for N-acetylhistamine are not standard, as the compound is primarily used as a biomarker. It may be used in cell culture models to study histamine metabolism and the effects of various treatments on histamine turnover.
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| Animal Protocol |
In vivo, N-acetylhistamine is a metabolite of histamine that is excreted in urine. Its levels in urine and plasma can be used as a biomarker for histamine turnover and anaphylactoid reactions. Animal studies may involve measuring N-acetylhistamine levels in response to allergic challenges or other stimuli.
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| ADME/Pharmacokinetics |
N-Acetylhistamine has a molecular weight of 153.18 g/mol and a molecular formula of C₇H₁₁N₃O. It is also known as Nω-acetylhistamine. The compound has a melting point of 147-149°C and a purity of ≥95%. It should be stored as a powder at -20°C for up to three years. Detailed pharmacokinetic parameters are not applicable as the compound is an endogenous metabolite.
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| Toxicity/Toxicokinetics |
N-Acetylhistamine is a primary metabolite of histamine formed through acetylation. It is an endogenous metabolite and is considered non-toxic at normal physiological levels. Elevated levels indicate increased histamine turnover rather than direct toxicity of the compound itself.
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| References | |
| Additional Infomation |
N-acetylhistamine is an acetamide compound with an acetyl group attached to the amino group on the side chain of the histamine molecule. It is a human metabolite, belonging to the acetamide and imidazole classes, and functionally related to histamine. N-acetylhistamine has been reported to exist in Drosophila melanogaster, Euglena, and other organisms with relevant data.
N-Acetylhistamine is a primary metabolite of histamine formed through acetylation during histidine metabolism. It is also known as Nω-acetylhistamine. The compound has gained attention as a sensitive and reliable biomarker for monitoring histamine turnover and anaphylactoid reactions. It is a member of the class of acetamides that is acetamide comprising histamine having an acetyl group attached to the side-chain amino function. Not approved for therapeutic use; intended for research and diagnostic purposes only. |
| Molecular Formula |
C7H11N3O
|
|---|---|
| Molecular Weight |
153.18174
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| Exact Mass |
153.09
|
| CAS # |
673-49-4
|
| PubChem CID |
69602
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| Appearance |
White to off-white solid powder
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| Density |
1.153g/cm3
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| Boiling Point |
501.7ºC at 760 mmHg
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| Melting Point |
147-149ºC(lit.)
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| Flash Point |
257.2ºC
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| Index of Refraction |
1.533
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| LogP |
0.479
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
2
|
| Rotatable Bond Count |
3
|
| Heavy Atom Count |
11
|
| Complexity |
138
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| Defined Atom Stereocenter Count |
0
|
| SMILES |
CC(NCCC1=CN=CN1)=O
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| InChi Key |
XJWPISBUKWZALE-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C7H11N3O/c1-6(11)9-3-2-7-4-8-5-10-7/h4-5H,2-3H2,1H3,(H,8,10)(H,9,11)
|
| Chemical Name |
N-[2-(1H-imidazol-5-yl)ethyl]acetamide
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| Synonyms |
N-Acetylhistamine; N-Omega-acetylhistamine
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ≥ 100 mg/mL (~652.8 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (16.32 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (16.32 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (16.32 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.5283 mL | 32.6413 mL | 65.2827 mL | |
| 5 mM | 1.3057 mL | 6.5283 mL | 13.0565 mL | |
| 10 mM | 0.6528 mL | 3.2641 mL | 6.5283 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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