| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
As a deuterium-labeled stable isotope, N-Acetylglycine-d5 does not have a specific pharmacological target. N-Acetylglycine itself is an endogenous metabolite involved in amino acid metabolism and is often used in biochemical studies to investigate metabolic pathways and enzyme kinetics. The deuterated form serves as an analytical tracer to study the metabolism and biological fate of N-acetylglycine in various biological systems.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
In vitro, N-Acetylglycine-d5 is used as a tracer to study glycine metabolism and acetylation pathways. The compound is incorporated into cell culture media to track the uptake and metabolism of N-acetylglycine. It does not exhibit intrinsic pharmacological activity but serves as a quantitative tool for studying cellular metabolism and enzyme kinetics. Stable heavy isotopes have been incorporated into drug molecules largely as tracers for quantitation during drug development. |
| ln Vivo |
In vivo, N-Acetylglycine-d5 is used in metabolic studies to trace the absorption, distribution, metabolism, and excretion of N-acetylglycine. The deuterium label allows for precise quantification of the compound and its metabolites in biological fluids and tissues using mass spectrometry. Deuteration may affect the pharmacokinetic and metabolic properties of the compound, which is an important consideration in tracer studies.
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| Enzyme Assay |
As an analytical standard, N-Acetylglycine-d5 is used in amino acid analysis and enzyme assays. Typical protocols involve spiking the labeled compound into samples prior to analysis by GC-MS or LC-MS. The compound serves as an internal standard to correct for matrix effects and instrument variability. The labeled compound co-elutes with the unlabeled analyte but is detected at a different mass-to-charge ratio, allowing for precise quantification.
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| Cell Assay |
In vitro cell culture experiments using N-Acetylglycine-d5 involve supplementing growth media with the labeled compound at defined concentrations. Cells are cultured for specified periods, after which the metabolism of the compound is analyzed by mass spectrometry. The compound can also be used to study glycine-related metabolic pathways and enzyme kinetics in various cell types.
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| Animal Protocol |
In vivo animal studies using N-Acetylglycine-d5 typically involve administering the compound to rodents via oral gavage or intravenous injection. Blood, urine, and tissue samples are collected at various time points to track the distribution and metabolism of N-acetylglycine. The deuterium label allows for precise quantification using mass spectrometry.
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| ADME/Pharmacokinetics |
N-Acetylglycine-d5 exhibits pharmacokinetic properties similar to those of unlabeled N-acetylglycine. As an amino acid derivative, it is absorbed from the gastrointestinal tract and distributed throughout the body. It is metabolized primarily in the liver and excreted in urine. The deuterium label provides a distinct mass shift for analytical detection without significantly altering the compound's physicochemical properties.
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| Toxicity/Toxicokinetics |
N-Acetylglycine-d5 is considered safe for research use at typical concentrations, as the unlabeled compound has no genotoxicity or acute toxicity. The compound is stable if stored under recommended conditions. As a stable isotope-labeled compound, it is not intended for therapeutic use and is handled under standard laboratory safety practices.
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| References | |
| Additional Infomation |
N-Acetylglycine-d5 is a stable isotope-labeled compound with a molecular formula of C₄H₂D₅NO₃ and a molecular weight of 122.13. Isotopic enrichment is typically 98 atom% D. The compound appears as a white to off-white solid and is stored at room temperature. It is primarily used as an internal standard for NMR, GC-MS, or LC-MS analysis and is valuable for biochemical studies investigating metabolic pathways and enzyme kinetics.
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| Molecular Formula |
C4H7NO3
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|---|---|
| Molecular Weight |
122.134089708328
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| Exact Mass |
122.074
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| CAS # |
1219805-82-9
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| Related CAS # |
N-Acetylglycine;543-24-8
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| PubChem CID |
131869422
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| Appearance |
White to off-white solid powder
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| LogP |
-1.2
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
8
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| Complexity |
110
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[2H]C([2H])([2H])C(=O)NC([2H])([2H])C(=O)O
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| InChi Key |
OKJIRPAQVSHGFK-ZBJDZAJPSA-N
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| InChi Code |
InChI=1S/C4H7NO3/c1-3(6)5-2-4(7)8/h2H2,1H3,(H,5,6)(H,7,8)/i1D3,2D2
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| Chemical Name |
2,2-dideuterio-2-[(2,2,2-trideuterioacetyl)amino]acetic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ~100 mg/mL (~818.8 mM; with ultrasonication)
H2O: ~50 mg/mL (~409.4 mM; with ultrasonication) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (20.47 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (20.47 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (20.47 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 8.1880 mL | 40.9400 mL | 81.8800 mL | |
| 5 mM | 1.6376 mL | 8.1880 mL | 16.3760 mL | |
| 10 mM | 0.8188 mL | 4.0940 mL | 8.1880 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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